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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
The effect of metribuzine on oxidative stress and antioxidant enzymes of signal crayfish
 LIDOVÁ, Jaroslava
The aim of this study was to investigate effects of the triazine herbicide metribuzine on oxidative stress level and antioxidant enzymes activity in gills, muscle and hepatopancreas of signal crayfish (Pacifastacus leniusculus Dana) and also extension of knowledge about effect of metribuzine on the environment. The experiment took up 60 days. Crayfish were exposured to metribuzine concentrations of 0.52 micrograms.l-1 (real environmental concentration) and 3.06 mg.l-1 (10% 96hLC50) for the first 30 days. Then a second phase followed depuration without metribuzine (30 days). Changes in the oxidative stress level (TBARS), superoxiddismutase (SOD) activity and catalase (CAT) activity were observed in all examined tissues. Changes in glutathionreductase (GR) activity were observed only in hepatopancreas. Chronic exposure of metribuzine demonstrated an oxidative damage of cell lipids, proteins and also changes in antioxidant activity in examined crayfish tissues.The results of this study suggest that crayfish are a very suitable organisms for toxicological tests and simultaneously extend knowledge about effect of metribuzine on the environment.
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The effects of prometryne on early life stages of common carp
 LIDOVÁ, Jaroslava
The effects of prometryne on early life stages of common carp Prometryne belongs to the triazine group of herbicides, which are used in agriculture. The aim of this study was to assess the impact of prometryne on mortality, occurrence of deformation, growth and development of early life stages of common carp (Cyprinus carpio L.). For assessing the impact of ryne on the early developmental stages of carp was used the method of embryo-larval toxicity test for fish (OECD test n. 210). Toxic effect of prometryne at four concentrations (0.51 micrograms.l-1, 80 micrograms.l-1, 1.2 mg.l-1 and 4 mg.l-1) was investigated during 35 days. Negative effects on mortality and growth were observed in the experimental group number four with the highest concentration of prometryne (4 mg.l-1). Retardation of ontogeny development of carp was observed in the experimental groups number three and four (concentrations 1.2 mg.l-1 and 4 mg.l-1). On the basic of test results was estimated at 35 day LC50 = 2,314 mg.l-1 prometryne. Based on mortality in experimental group was estimated LOEC = 1,10 mg.l-1 and NOEC = 0,85 mg.l-1 prometryne.
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