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Ways to test for Helicobacter and calprotectin
 LAVIČKOVÁ, Lucie
H. pylori is a gram-negative rod bacteria of a spiral shape. It is obligatory microaerofile. It has strong urease activity. It colonize the stomach mucosa and it is proved, that almost the half of human population is colonized. H. pylori concluding other factors can cause gastritis which can become chronic. Other diseases can be influenced by chronic gastritis - for example: gastroduodenal ulcer disease and stomach adenocarcinoma. H. pylori is concerned to be carcinogen of the first class. Calprotectin is a cytosol protein. It gradually releases from activated or lytic leucocytes. It is made of monocytes and neutrophiles. Calprotectin concentration in in faecal specimen correlates with the amount of leucocytes in intestine's lumen. This finding allows doctors to estimate the amount of intestine mucosas' infiltration by leucocytes according to evaluation of calprotectin levels. Calprotectin is highly sensitive to the presence of intestine inflammation, so it is a proper indicator of unspecific intestine inflammation diagnostics. The main aim of my bachelor thesis is to become familiar with identification methods of H. pylori and calprotectin in labs. Moreover, I aimed to become familiar with proper results interpretation of used methods. I estimated a hypothesis and an experimental question. In the theoretical part I focused on contemporary knowledge about H. pylori a calprotectin. I described common diagnostic methods, illnesses they cause and their treatment. The experimental part took place at Microbiology department of Stafila spol. sro laboratories. I examined the presence of H. pylori in faecal specimen by DIMA method. I determined the calprotectin levels by BUHLMANN method. I personally worked with 25 samples. I did my work in guidance of MUDr. Cihlová and her colleagues. I statistically examine my results. I made statistics of H. pylori frequency and determined the hypothesis. I also made statistic of negative, positive and strongly positive numerosity levels of calprotectin in faecal specimen and found the answer to my question.
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2 LAVIČKOVÁ, Lenka
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