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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Drop coating deposition Raman spectroscopy of biologically important molecules
 Kuižová, Alžbeta ; Kočišová, Eva (advisor) ; Holoubek, Aleš (referee)
Drop coating deposition Raman (DCDR) spectroscopy is a special method of Raman spectroscopy, which is based on the evaporation of solvent from a drop of solution or suspension on a hydrophobic surface. This typically leads to the formation of ring-shaped drying pattern, often called as "coffee ring". As a result a preconcentration of a material and higher intensity of Raman signal in comparison with Raman scattering from solution is obtained. In this work several hydrophobic surfaces with different roughness and hydrophobicity were compared: a smooth substrate with polytetrafluorethylen (pPTFE) coating and nanorough substrates where surface hydrophobicity was formed by deposited cupper or argent nanoparticles with different concentration. It was shown that for DPPC liposome suspension stronger preconcentration is obtained by means of a nanorough substrate. When different nanorough substrate compared, no better improvement was acquired. As for the drying of drops at different temperatures (from 15řC to 60řC) deposited on the smooth pPTFE substrate and the substrate with argent nanoparticles, it was observed that Raman spectra did not reveal any spectral changes corresponding to phase transition of lipid. In case of drying at temperatures higher than a temperature of the phase transition, non-homogeneities...
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Preparation and characterization of photovoltaic cells based on photosystem I
 Kuižová, Alžbeta ; Pšenčík, Jakub (advisor) ; Alster, Jan (referee)
Photosystem I is increasingly used for new types of photovoltaic devices because of high stability. The goal of this thesis was to characterize organic photovoltaic cells based on photosystem I. Our task was to compare three electron mediators, namely TMPD, DCIP and cytochome c, and to choose the best one. In the experimental part of thesis, TMPD was selected as the most effective electron mediator. Subsequent measurements were performed to optimize the thickness of the cell and the concentration of photosystem I and TMPD in a final solution. Solar cells have been characterized by absorption and action spectra, and current-voltage characteristics were used to determine the overall power. It was found out that thinner photovoltaic cells work better and that the use of more concentrated solutions of photosystem I and TMPD leads to more efficient cells.
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