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National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
Charakteristika různorodých půd a sedimentů užitím specifických aktinomycetových r-DNA PCR primerů
 Hill, P. ; Krištůfek, Václav ; Caballero, S. ; Kroetsch, D. J. ; Rauch, Ota
Most molecular biological studies of the soil characterise a few soils or sediments under a range of treatments or over a limited time. It is difficult to compare results between studies due to variations in DNA extraction method and PCR conditions. High diversity of the soil bacterial population means that it is impossible to characterise many soils through cloning and sequencing and that finger prints are often blurred and difficult to interpret. We compare a broad range of soils and zoo/anthropogenic sediments with actinomycete specific primers. ARDRA and cloning showed that these primers amplified mainly verrucomicrobia from tropical, temperate and sub-arctic soils and actinomycetes from biologically active and urban street sediments. We found two new groups that were broadly distributed in soils: a new actinomycete family and the AD-3 candidate division that was found in acid tropical and sub-arctic soils
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