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Vývoj imunochromatografického stanovení pro testování reálných vzorků obsahujících TNT
Kosařová, Lucie ; Chocholouš, Petr (advisor) ; Chocholoušová Havlíková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate: Lucie Kosařová Supervisor: PharmDr. Petr Chocholouš, Ph.D. Title of diploma thesis: Development of lateral flow immunoassay for testing real samples containing TNT Obtaining results very fast is requested more often these days. This is particularly true for dangerous and potentially dangerous compounds. One of these compounds is 2,4,6,-trinitrotoluene (TNT), which was tested in this research. TNT has been extensively used as a military explosive and is still one of the most widely used explosives. Its advantages, such as low manufacturing costs, safety of handling and fairly good explosive power can be tempting for abuse by terrorists. In addition, TNT is a well known pollutant because of its toxicity and low biodegradability. It can contaminate surface and ground waters, soils and sediments, thus causing environmental and health problems. According to these facts there is a requirement to detect this nitroaromatic compound in minimal concentrations very fast and outside the laboratory. These demands could be met by the lateral flow test. In this research lateral flow immunoassay (LFIA) based on colloidal gold nanoparticle labels was developed for detection of TNT in real samples. The...
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Determination of estrogen derivatives by sequential injection chromatography
Kosařová, Lucie ; Chocholouš, Petr (advisor) ; Chocholoušová Havlíková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate: Mgr. Lucie Kosařová Consultant: PharmDr. Petr Chocholouš, Ph.D. Title of Thesis: Determination of estrogen derivatives by sequential injection chromatography This work presents the first utilization of sequential injection chromatography for determination of four estrogen derivatives with similar structure and an internal standard (ethylparaben) with the use of monolithic column Merck® Chromolith® Performance RP-18e 100 mm × 3 mm and pre-column Merck® Chromolith® RP-18e 5 mm × 3 mm of total length 105 mm. A commercially available SIC system - SIChromTM (FIAlab® Instruments Inc., USA), mobile phase composed of acetonitrile:water 40:60 (v/v) and CCD UV-VIS detector were used. Spectrophotometric detection was set up at wavelength of 225 nm. The injected volume of sample solutions was 10 µl and 3 ml of mobile phase was pumped isocratic at flow rate 8 μl.s-1 . High separation efficiency, good peak symmetry and resolution were obtained at these conditions, showing another possibility of use sequential injection chromatography in analytical chemistry. Keywords: sequention injection chromatography, monolithic columns, estrogens
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Vývoj imunochromatografického stanovení pro testování reálných vzorků obsahujících TNT
Kosařová, Lucie ; Chocholouš, Petr (advisor) ; Chocholoušová Havlíková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate: Lucie Kosařová Supervisor: PharmDr. Petr Chocholouš, Ph.D. Title of diploma thesis: Development of lateral flow immunoassay for testing real samples containing TNT Obtaining results very fast is requested more often these days. This is particularly true for dangerous and potentially dangerous compounds. One of these compounds is 2,4,6,-trinitrotoluene (TNT), which was tested in this research. TNT has been extensively used as a military explosive and is still one of the most widely used explosives. Its advantages, such as low manufacturing costs, safety of handling and fairly good explosive power can be tempting for abuse by terrorists. In addition, TNT is a well known pollutant because of its toxicity and low biodegradability. It can contaminate surface and ground waters, soils and sediments, thus causing environmental and health problems. According to these facts there is a requirement to detect this nitroaromatic compound in minimal concentrations very fast and outside the laboratory. These demands could be met by the lateral flow test. In this research lateral flow immunoassay (LFIA) based on colloidal gold nanoparticle labels was developed for detection of TNT in real samples. The...
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