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Determination of Sibutramine in Pharmaceutical Preparations by Capillary Zone Electrophoresis
Korejsová, Tereza ; Pospíšilová, Marie (advisor) ; Polášek, Miroslav (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytická chemie Candidate Mgr. Tereza Korejsová Consultant doc. RNDr. Marie Pospíšilová, CSc Title of Thesis Stanovení sibutraminu ve farmaceutických přípravcích metodou kapilární zónové elektroforézy A simple capillary zone electrophoresis (CZE) with sulfated β - CD as chiral selector has been developed for separation and quantitative analysis of R - and S - sibutramine (SIB). The method was used for the assay of racemic sibutramine in pharmaceutical preparations Meridia®, Lindaxa®, Sibutramin Sandoz® and Sibutramin Teva®. The separation was optimized by testing the parameters such as buffer type and its concentration, buffer pH, type and concentration of chiral selectors, separation temperature and voltage. The analyses were carried out using a polyacrylamide-coated capillary (PAA) with total length 30 cm and 50 µm ID. Optimum conditions for separation of the enantiomers of sibutramine were achieved with 100 mM phosphate buffer of pH 3,0 (adjusted by TRIS) containing 2,0 mM sulfated β - CD. The applied voltage was 15 kV and the capillary temperature was kept constant at 180 C. The total analysis time took about 5 - 6 minutes. Regression equation showed good linear relationships in the range 5 - 250 µg/ml of each...
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Determination of Sibutramine in Pharmaceutical Preparations by Capillary Zone Electrophoresis
Korejsová, Tereza ; Pospíšilová, Marie (advisor) ; Polášek, Miroslav (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytická chemie Candidate Mgr. Tereza Korejsová Consultant doc. RNDr. Marie Pospíšilová, CSc Title of Thesis Stanovení sibutraminu ve farmaceutických přípravcích metodou kapilární zónové elektroforézy A simple capillary zone electrophoresis (CZE) with sulfated β - CD as chiral selector has been developed for separation and quantitative analysis of R - and S - sibutramine (SIB). The method was used for the assay of racemic sibutramine in pharmaceutical preparations Meridia®, Lindaxa®, Sibutramin Sandoz® and Sibutramin Teva®. The separation was optimized by testing the parameters such as buffer type and its concentration, buffer pH, type and concentration of chiral selectors, separation temperature and voltage. The analyses were carried out using a polyacrylamide-coated capillary (PAA) with total length 30 cm and 50 µm ID. Optimum conditions for separation of the enantiomers of sibutramine were achieved with 100 mM phosphate buffer of pH 3,0 (adjusted by TRIS) containing 2,0 mM sulfated β - CD. The applied voltage was 15 kV and the capillary temperature was kept constant at 180 C. The total analysis time took about 5 - 6 minutes. Regression equation showed good linear relationships in the range 5 - 250 µg/ml of each...
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