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Creation tetraploid varieties of the species Phlox paniculata in-vitro
Konopíková, Alžběta ; Matiska, Pavel (advisor) ; Sedlák, Petr (referee)
Phlox paniculata belongs to the group of the most important perennials and it often appears in the range of gardens and parks. Creation of tetraploid varieties of this species is important capitally from a breeding point of view, they use them mainly because of larger proportions of tetraploid cells and because of the larger number of chromosomes and greater variability in the further multiplication. In my bachelor thesis, I examined the possibility of induction of tetraploid varieties of the species Phlox paniculata with target to determine the best combination of plant-growth regulators, supporting regeneration and chemomutagens inducing tetraploids. As a plant material were chosen leafs segments size of ca 1 cm2, their cultivation passed of on MS medium. Cultivation of experiments carried throughout in an incubator with controlled temperature and photoperiod. They were investigated the effects of auxin IAA (indole-3-acetic acid) and cytokinin BAP (6-benzylaminopurine) and TDZ (thidiazuron) to regeneration of leaf segments. They were also investigated the effects of chemomutagens colchicine and oryzalin to creation of tetraploids. The best results of regeneration were obtained with the combination of MS medium with TDZ 3 mg . l-1 and IAA 1 mg . l-1, in this case control variant of this medium reached 100 % regeneration of leaf segments. As less suitable chemomutagen for this thesis showed colchicine, in this case was a 100 % mortality of leaf segments cultivar 'Starfire'. On the other side as more effective herbicide showed oryzalin at concentration 14 mg . l-1 applied for 14 days, in this variant was the percentage of recovery 18,6 %. Subsequent regeneration of explants after effect of chemomutagens is time-consuming, and therefore has not been possible to verify the actual amount of potentially obtained tetraploids yet . This topic will be the subject of follow-up thesis of another student.
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