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Vliv železa a jeho bioakumulace v sinici Spirulina a mikrořase Chlorella
 Koláčková, Kristýna
Iron is a trace element playing a key role in a number of metabolic pathways important for all living organisms. Iron deficiency is one of the most widespread problems in human nutrition. In order to possibly use Spirulina and Chlorella as dietary supplements for people suffering from iron deficiency sideropenic anemia, the effect of different concentrations of iron in the medium (0 mg/L, 5 mg/L and 10 mg/L) on its amount in the biomass was investigated. The optimal amount of Fe2+ in the medium was determined using spectrophotometric determination of optical density (OD) and iron content in Arthrospira maxima and Chlorella vulgaris biomass, which was determined by atomic absorption spectrometry (AAS). Expression analysis, performed in a model organism with a sequenced whole genome, Chlamydomonas reinhardtii, revealed the effect of selected concentrations of Fe2+ on the genes of the oxidative stress regulatory pathway. The results of the above analyzes show what are the optimal levels of iron cations for the growth of the studied organisms and its accumulation in them. The effect of iron in the chosen form, its assimilation and bioaccumulation in the cyanobacteria Spirulina and Chlorella microalgae was thus clarified.
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4 Koláčková, Kateřina
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