National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Podnikání v agrobyznysu v zázemí velkého města: případová studie Kuřimsko
Kočková, Lucie
The aim of this thesis consists in an evaluation of agribusiness in the microregion and in identification of problems in agribusinesses issues that some of the chosen companies operating in the agrarian sector may face. The thesis is divided into three parts. The first part defines basic concepts. The second part focuses directly on the microregion itself and further describes individual municipalities (Chudčice, Moravské Knínice, Veverská Bítýška and Kuřim). The last part involves analysis of the chosen companies. The sociological methods were used to ask questions regarding their current business situation. Individual representatives had expressed that the situation is gradually becoming more serious because of the continuous decrease in the agricultural land due to the conquest of the land. Another difficulty that the farmers face is the government granting; the farmers are not able swiftly to adapt to the constant changes.
Molecular and biochemical characteristics of genetically modified barley plants
KOCKOVÁ, Lucie
Modern agriculture often employs broad-spectrum herbicides combined with herbicide-resistant crops. Usually, this is achieved by altering the crop genom (genetic modification) by inserting of a specific gene coding for resistance to specific herbicide or group of herbicides. Apllying such herbicide thus results in minor crop damage. The resistence of crops against broad-spectrum herbicides depends on the genes, which were inserted into the genom. The gene bar is often used as a resistence -providing element. It mediates resistance against a broad spectrum of herbicides, such as glufosinate and Bialahops. For the selection of transformants and preliminary assessment of the target transgen expression level a suitable marker gene is simultaneously inserted. For this purpose, gene for bacterial ??glucuronidase (GUS) is often used. It is considered to be one of the most frequently used reporter systems for the assessment of transgen expression and also allows to analyze the expression on the tissue, cell and whole cell organelles levels. In this diploma thesis the PCR method and histochemical detection of the enzyme glucuronidase presence were used to detect and evaluate transgenic plants of cv. Golden Promise spring barley, modified by genes bar and gus. The presence of gus gene was determined in different parts of plants.
Utilization of PCR-RFLP approach for evaluation of ITS polymorphism in selected \kur{Beauveria bassiana} strains/isolates
KOCKOVÁ, Lucie
The aim of this study was the evaluation of the suitability PCR-RFLP technique and ITS region analysis for determination the genetic variability of the entomopathogenic fungi - Beauveria bassiana, Beauveria brongniartii and Isaria fumosorosea. By the means of ITS-RFLP technique, it was possible to identify the uniformity of the B. bassiana isolates from NP Šumava, the higher level of polymorphism of B.bassiana isolates from different geographical areas was detected and it was possible to distinguish different entomopathogenic fungi used in this study.

See also: similar author names
3 KOCKOVÁ, Lucie
1 KOČKOVÁ, Lenka
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