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Effect of selected antineoplastic drugs on the gene expression of DNA repair proteins
Klčová, Silvia ; Jirkovská, Anna (advisor) ; Suchá, Simona (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Silvia Klčová Supervisor: PharmDr. Anna Jirkovská, Ph.D. Title of diploma thesis: Effect of selected antineoplastic drugs on the gene expression of DNA repair proteins. Every single cell of the human body is continuously exposed to a wide range of stress factors, with the consequence of damage to the DNA molecule. The resulting changes represent variety of alterations - from simple alkylation modifications of bases to the most unfavorable double-strand breaks (DSBs). However, the effect of cellular stress and subsequent genotoxic DNA damage is a double-edged sword. On the one hand, typical alterations in the genome can be triggered by mutagenic agents (such as components of tobacco smoke or ionizing radiation). Consequences of their action can accumulate and trigger loss of control over various steps of the cell cycle, which results in tumor cell transformation. On the other hand, however, inducing detrimental impact affecting the genome of tumor cells is one of the fundamental approaches in cytostatic treatment of cancer. Therefore, we focused our research on several antineoplastic drugs widely used in clinical practice (etoposide, daunorubicin, dexrazoxane) or undergoing clinical...
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Osteoporosis - Current trends in diagnostics and therapy
Klčová, Silvia ; Kudláčková, Zděnka (advisor) ; Němečková, Ivana (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Author: Silvia Klčová Thesis supervisor: PhDr. Zděnka Kudláčková, Ph.D. Form: Bachelor thesis Study programme: Medical bioanalytics Thesis title: Osteoporosis - Current trends in diagnostics and therapy Background: This bachelor thesis is focused on elucidating the problem of osteoporosis. The aim of this work is to summarize current knowledge about this disease, especially about the methods of its diagnosis and therapy. Main findings: Osteoporosis is a progressive metabolic bone disease that is based on an imbalance in bone remodeling. This disease is characterized by a loss of bone tissue and the formation of porous bones, that are prone to fracture. Fractures are the most serious complication of osteoporosis. This thesis describes in detail the currently used diagnostic methods and therapeutic principles. Recognition of the presence of osteoporosis and subsequent initiation of therapeutic measures is essential for the management of this condition. There are several instrumental and laboratory methods used for diagnosis, which are based on different principles. Pharmacotherapy affecting bone remodeling, by inducing changes in the presence or functionality of executive bone cells, is...
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