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National Repository of Grey Literature

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	The role of caspases in mammalian cell migration Kasalová, Lenka ; Rösel, Daniel (advisor) ; Hovorka, Ondřej (referee) Detailed record
	In-vitro analysis of amoeboid-mesenchymal transition of A375m2 melanoma cells Kasalová, Lenka ; Rösel, Daniel (advisor) ; Vomastek, Tomáš (referee) The invasion of cancer cells is an important aspect of cancer progression. Single tumor cells exhibit at least two types of invasion in 3D environment, mesenchymal and amoeboid invasion. Tumor cells can switch between these two modes of movement depending on cellular status and surrounding environment. Amoeboid-mesenchymal transition (AMT) is less explored then mesenchymal-amoeboid transition (MAT). We performed a proteomic analysis of amoeboid-mesenchymal transition of human melanoma cell line A375M2. We have induced amoeboid-mesenchymal transition by treatment with a ROCK inhibitor Y27632 in 3D matrigel matrices and in 2D environment. Induction of the amoeboid-mesenchymal transition has changed a level of expression of 92 proteins and a level of phosphorylation of 15 proteins. Expression of only 17 proteins and phosphorylation of 8 proteins was identically changed in both of these environments. We found that PKCα regulates amoeboid migration and that treatment of cells with a PKCα inhibitor Gö6976 induces amoeboid-mesenchymal transition. Analysis of the proteomics data have further shown that induction of AMT by the ROCK inhibitor Y27632 leads to activation of antiapoptotic signals and activation of signaling pathways involved in regulation of actin cytoskeleton especially regulation of focal... Detailed record
	The role of caspases in mammalian cell migration Kasalová, Lenka ; Hovorka, Ondřej (referee) ; Rösel, Daniel (advisor) Detailed record

See also: similar author names
1	Kasalová, Lucie

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