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Influence of extracellular matrix environment on gene expression in liver myofibriloblasts
Jiroutová, Alena ; Kanta, Jiří (advisor) ; Ehrmann, Jiří (referee) ; Sedláček, Radislav (referee)
Influence of extracellular matrix environment on gene expression in liver myofibroblast (summary) Hepatic stellate cells (HSC) and liver myofibroblasts (MF) are two cell populations most likely responsible for the synthesis of majority of connective tissue components in fibrotic liver. They differ in their origin and location in the liver, and in the spectrum of genes they express. HSC are located in Disse spaces of normal rat liver around the sinusoids, in fibrotic liver they become activated, proliferate and they undergo transdifferentiation into myofibroblast-like cells. Myofibroblasts are heterogenous cell population that consists at least of portal pMF, septal sMF and interface iMF. pMF, which are adjacent to bile duct epithelia, may be a mediator of billiary type fibrosis. sMF are located within and along the collagenous septum in cirrhotic liver. Little is known about the expression of genes involved in connective tissue metabolism in MF cultured in fibrin or collagen gels that more closely resemble natural cell environment. Fibrin is deposited in liver at sites of injury and collagen type I forms a substantial part of fibrotic septa. In our study oligo cDNA array analysis was used to determine gene expression in quiescent HSC, activated HSC and MF isolated from both normal and CCl4-cirrhotic liver....
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Wound repair and diabetic wound defects
Slavkovský, Rastislav ; Kanta, Jiří (advisor) ; Dvořánková, Barbora (referee) ; Veverková, Lenka (referee)
of dissertation thesis WOUND REPAIR AND DIABETIC WOUND DEFECTS. Rastislav Slavkovský Impaired diabetic wound healing is an important current medical issue, mainly concerning patients recovering from complicated operations or patients with ulcers on their feet. Hyaluronan (HA) plays an important role in the repair of damaged skin and has been used for the treatment of wounds. A mixture of high molecular weight HA with the antiseptic iodine complex KI3 (Hyiodine) was reported to accelerate wound healing in patients with diabetes and patients after surgery. We investigated how this mixture affects wound contraction, granulation tissue (GT) and wound edges in excision skin wounds in rats. Hyiodine was applied to full- thickness wounds made on the back of rats. The areas of the contracting wounds were calculated from digital photographs. The migrating edges of the wound were studied by histological methods. The properties of GT were studied in wounds in which contraction was prevented by the insertion of plastic rings. The effects of Hyiodine were compared with those of high molecular weight HA, low molecular weight HA and KI3 solution. Hyiodine accelerated wound contraction significantly in the first 5 days of healing. On day 3, Hyiodine-treated wounds had reduced to 63% of the original area, whereas...
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Wound repair and diabetic wound defects
Slavkovský, Rastislav ; Kanta, Jiří (advisor) ; Dvořánková, Barbora (referee) ; Veverková, Lenka (referee)
of dissertation thesis WOUND REPAIR AND DIABETIC WOUND DEFECTS. Rastislav Slavkovský Impaired diabetic wound healing is an important current medical issue, mainly concerning patients recovering from complicated operations or patients with ulcers on their feet. Hyaluronan (HA) plays an important role in the repair of damaged skin and has been used for the treatment of wounds. A mixture of high molecular weight HA with the antiseptic iodine complex KI3 (Hyiodine) was reported to accelerate wound healing in patients with diabetes and patients after surgery. We investigated how this mixture affects wound contraction, granulation tissue (GT) and wound edges in excision skin wounds in rats. Hyiodine was applied to full- thickness wounds made on the back of rats. The areas of the contracting wounds were calculated from digital photographs. The migrating edges of the wound were studied by histological methods. The properties of GT were studied in wounds in which contraction was prevented by the insertion of plastic rings. The effects of Hyiodine were compared with those of high molecular weight HA, low molecular weight HA and KI3 solution. Hyiodine accelerated wound contraction significantly in the first 5 days of healing. On day 3, Hyiodine-treated wounds had reduced to 63% of the original area, whereas...
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General features of hepatic stellate cells undergoing apoptosis in cirrhotic rat liver
Bittnerová, Lenka ; Kanta, Jiří (advisor) ; Dršata, Jaroslav (referee) ; Krtek, Vladislav (referee)
Liver fibrosis represents a significant worldwide health problem. It is a response of liver to repeated injury and it is characterized by breakdown of normal extracellular matrix (ECM) resembling basement membrane in composition and by accumulation of ECM containing fibrillar type I collagen. Although more than one potential source of ECM exist, the largest part of connective tissue components is synthesized by activated hepatic stellate cells (HSC). Hepatic stellate cells are located in the space of Disse between endothelial cells and parenchymal cells and their main function is the uptake and storage of vitamin A and other retinoids. Inducing or accelerating their apoptosis is a potential way of liver fibrosis treatment. Aim of our study was to find out how collagen gell influences HSC as the effect of ECM on HSC apoptosis has not been studied yet. We have used gel made of type I collagen, the main component of fibrotic liver ECM, to study how it affects spontaneous apoptosis of HSC isolated from carbon tetrachloride damaged liver and the apoptosis of normal HSC exposed to apoptosis inducing agents, gliotoxin, cycloheximide and cytochalasin D, in vitro. HSC were prepared by perfusion of rat normal and cirrhotic liver with pronase and collagenase solutions, followed by centrifugation of the cell...
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Influence of extracellular matrix environment on gene expression in liver myofibriloblasts
Jiroutová, Alena ; Kanta, Jiří (advisor) ; Ehrmann, Jiří (referee) ; Sedláček, Radislav (referee)
Influence of extracellular matrix environment on gene expression in liver myofibroblast (summary) Hepatic stellate cells (HSC) and liver myofibroblasts (MF) are two cell populations most likely responsible for the synthesis of majority of connective tissue components in fibrotic liver. They differ in their origin and location in the liver, and in the spectrum of genes they express. HSC are located in Disse spaces of normal rat liver around the sinusoids, in fibrotic liver they become activated, proliferate and they undergo transdifferentiation into myofibroblast-like cells. Myofibroblasts are heterogenous cell population that consists at least of portal pMF, septal sMF and interface iMF. pMF, which are adjacent to bile duct epithelia, may be a mediator of billiary type fibrosis. sMF are located within and along the collagenous septum in cirrhotic liver. Little is known about the expression of genes involved in connective tissue metabolism in MF cultured in fibrin or collagen gels that more closely resemble natural cell environment. Fibrin is deposited in liver at sites of injury and collagen type I forms a substantial part of fibrotic septa. In our study oligo cDNA array analysis was used to determine gene expression in quiescent HSC, activated HSC and MF isolated from both normal and CCl4-cirrhotic liver....
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