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The effect of flubendazole on proliferation, migration and adhesion of oral cancer cells in vitro Kamarýtová, Jana ; Skálová, Lenka (advisor) ; Szotáková, Barbora (referee) Charles Univerzity in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Jana Kamarýtová Supervisor: prof. RNDr. Lenka Skálová, Ph.D. Supervizor specialist: RNDr. Věra Králová, Ph.D. Title of diploma thesis: Impact of flubendazol on proliferation, migration and adhesion of cells of oral carcinoma in vitro Flubendazol (FLU) belongs to the group of benzimidazole anthelmintics and is widely used for treatment of parasitic diseases in human and veterinary medicine. Molecular mechanism of FLU action consists in inhibition of protein β-tubulin subunits. It interferes with microtubule polymerization, eventually causing cell damage. FLU also inhibits the energetic metabolism of parasite cell which leads gradually to the death of the parasite. The ability to disrupt the production of microtubules leads to a theoretical possibility to use FLU and other benzimidazole anthelmintics in cancertreatment. In this thesis we studied the impact of FLU on proliferation, migration and adhesion of oral squamous carcinoma cells in vitro. For our experiment we used two cell lines derived from oral squamous carcinoma DOK and PE/CA-PJ15 and cell line GF derived from gingiva of a healthy human donor. We treated cells by FLU in concentration range from 0,01 to 10 µM and after 72-hour... Detailed record

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2	Kamarýtová, Jitka

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