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Vliv lipopolysacharidu a muramyl dipeptidu na lymfocyty mléčné žlázy jalovic
Kabourková, Eliška
The objective of this study was to get a better knowledge of lymphocyte apoptosis, surface expression of CD44 on lymphocytes and infammatory cytokine production (TNF-alfa, IL-4, IL-10, and TGFbeta 1) throughout inflammatory response of bovine mammary gland caused by bacterial toxin lipopolysacharide (LPS) and muramyl dipeptide as a structural unit of peptidoglycan (MDP). In the experiments, there were used LPS and MDP at the specified concentrations (5 microg and 500 microg, respectively). The inflammatory response was analysed at selected time points (24, 48, 72, and 168 h) following stimulation of the mammmary gland. As a control, the same volumes of physiological saline solution was used. The lymphocyte apoptosis and the surface expression of the CD44 on the lymphocytes were detected in the mammary gland lavages collected from the 8 clinically healthy virgin Holstein x Czech Pied heifers by flow cytometry. Further, it was analysed the concentrations of the cytokines at the same selected time points by an ELISA method. The itramammary instillation using LPS and MDP resulted in the highest lymphocyte apoptosis 48 hours after the inductor challenge. The receptor CD44 was highly correlated with the lymphocyte apoptosis occurrence. It is likely that CD44 receptor is present throughout lymphocyte apoptosis process. The cytokine concentrations obtained indicate that the cytokines may play a role in apoptosis during mammary gland infammation.
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Poruchy příjmu potravy u žáků středních škol
Kabourková, Eliška
The bacherol thesis deals with eating disorders of a high school pupil based on the qualitative research. The theoretical part describes the current knowledge of eating disorders, the segmentation, the specification, the causes, the diagnosis, the possibilities of the treatment and the prevention of eating disorders. The attitude of the pupil's high school and the family is pointed out as well. The method of the practical part is based on the searching and studying of the professional literary sources and the information is handling into the review abstracting. The aim of the thesis is to get the details from the high school pupil who suffers by eating disorders and find out her relation approaches to her school, her family and her neighbourhood influenced by the eating disorders. The method of the practical part is the case study of the high school pupil who suffers by the bulimia nervosa. The data are processed by the qualitative analysis using the casuistry. The recommendations for the educational and psychological practice are suggested following the research results.
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