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Immunohistochemical analysis of the effect of M1043 on endoglin expression in a mouse model of NASH
Kubíčková, Adéla ; Němečková, Ivana (advisor) ; Kudláčková, Zděnka (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Biological and Medical Sciences Candidate: Adéla Kubíčková Supervisor of Bachelor thesis: RNDr. Ivana Němečková, Ph.D. Title of Bachelor thesis: Immunohistochemical analysis of the effect of M1043 on endoglin expression in a mouse model of NASH The morphological and functional changes of the endothelial cells of the hepatic sinusoids and endothelial dysfunction occur during nonalcoholic steatohepatitis (NASH). It has been shown that the expression of adhesion molecules (VCAM-1, ICAM-1) and endoglin increase in NASH. Endoglin affects the activation of hepatic stellate cells, which attenuate the process of fibrotization. The aim of this bachelor thesis was to detect the expression of endoglin and the adhesion molecule VCAM-1 in a mouse model of NASH after 8 weeks of CDAA diet (choline-deficient and L-amino acid-defined diet). Mice were divided into 2 groups (n=8). The control group was injected with rat IgG antibody (CDAA+rat IgG group) for 4 weeks and the CDAA+M1043 group had anti-endoglin antibody M1043. The analysis of selected proteins expression was performed by immunohistochemical method using avidin-biotin method. The results of this thesis show that there was not any reduction in the expression of VCAM- 1 or...
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Leisure healthy siblings and their participation in family life with a sibling with a disability
KUBÍČKOVÁ, Adéla
This diploma thesis deals with the position, leisure time and life of a healthy sibling in a family which takes care of a family member with disabilities. The theoretical part is divided in four chapters, which are aimed at a family, a family with a disabled member, healthy siblings, constellations between siblings, the definition of leisure time, particular developmental psychological characteristics of healthy siblings and disabled siblings and also the actual division of disability. The practical part is based on qualitative research. Due to the semi-structured interviews it attempts to differentiate how the quality of leisure time and especially their own personal life is perceived by healthy siblings themselves.
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Respite care for individuals caring for a disabled family member.
KUBÍČKOVÁ, Adéla
The bachelor thesis is divided into two parts, theoretical and practical. The theoretical part is composed of individual chapters and their subheads. The first chapter focuses on disability, its technical summary and division. The following chapter deals with a situation of a family into a disabled child is born and describes a model of mental crisis affecting parents who face this fact. The final chapter is focused on respite care itself, its division, and outlines particular activities and acts offered by respite care. The aim of the practical part was to find out if the families who care of their disabled member are aware of the respite care services and if they have an opportunity to take advantage of them. The partial aims of the thesis try to find out if parents make use of respite care services in their neighbourhood and what ways of relaxing the home caring family members have. Three basic reserch questions were set. 1. How much are the families burdened with the care of their disabled member? 2. Do the parents know any institutions in their neighborhood providing respite care services for caring families? 3. Do the home caring parents have an opportunity to take advantage of an institution with respite care services? A qualitative research was chosen to work out the practical part of the thesis. A method of inquiries, namely interviews, was selected to collect a particular item of information. In the form of half-structured interviews the research was realized between january and april 2015. Purposefully, families with a disabled family member were chosen from the South-bohemian region, namely from Soběslav and Tábor surroundings. Most of the researched families have other healthy offsprings and only two of them are complete. One of the interviews was carried out with a grandparent. In the other four families mothers who have a disabled child in the age from seventeen to twenty-four were interviewed. The fact is, that usually mothers stay with these children at home and play the biggest part in the care. I have chosen these respondents mainly due to the fact that I worked with their children as a volunteer. They have become my friends in course of the time. My mother was one of the respondents, too. In the final part, the cases interpretation focus on introducing the particular families and then the outcome report got from the interviews with family members, mothers in particular, follows. The research tends to show that the home caring families would like to take advantage of respite care services as the burden is enormous. Although the families are aware of the usefulness of respite care, they do not know any institutions offering this kind of services. Another findings concern ways of relaxing in home care families. They try to save time for themselves and find ways of relaxing. This bachelor thesis could serve as a source of information for the students, home caring families and social and health care workers.
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