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Influence of acellular pig dermis on the healing of thermal skin injuries
Zajíček, Robert ; Königová, Radana (advisor) ; Brychta, Pavel (referee) ; Klein, Leo (referee)
The submitted dissertation addresses the issue of acellular pig dermis in healing burn wound. The acellular xenodermis Xe-Derma® (XD) has been developed in the Prague Burn Centre in cooperation with the Czech Academy of Sciences and has been produced by a Czech company. Several culture techniques for cultivation of human primary keratinocytes were tested. The bioactivity of XD was studied by a cell culture assay in an organotypic culture system at the air-liquid interface. We compared keratinocyte proliferation and formation of epidermis in vitro with formation of neo-epidermis in the deep dermal wounds treated with XD (in vivo). Morphological and immunohistochemical analysis of keratinocytes confirmed the similarity of organization and differentiation of the cultured epidermal cells to the normal epidermis. Ability to stimulate growth and differentiation of keratinocytes connote bioactivity of XD. The natural structure and firmness of XD (composed of collagen and elastic fibres with the surface responding to the basement membrane) enables adhesion,proliferation, migration and differentiation of epidermal cells. In the clinical use XD appertains to temporary covers in burn treatment, particularly on scalds in children. In the frame of grant project IGA there has been investigated healing of widely meshed...
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Influence of acellular pig dermis on the healing of thermal skin injuries
Zajíček, Robert ; Königová, Radana (advisor) ; Brychta, Pavel (referee) ; Klein, Leo (referee)
The submitted dissertation addresses the issue of acellular pig dermis in healing burn wound. The acellular xenodermis Xe-Derma® (XD) has been developed in the Prague Burn Centre in cooperation with the Czech Academy of Sciences and has been produced by a Czech company. Several culture techniques for cultivation of human primary keratinocytes were tested. The bioactivity of XD was studied by a cell culture assay in an organotypic culture system at the air-liquid interface. We compared keratinocyte proliferation and formation of epidermis in vitro with formation of neo-epidermis in the deep dermal wounds treated with XD (in vivo). Morphological and immunohistochemical analysis of keratinocytes confirmed the similarity of organization and differentiation of the cultured epidermal cells to the normal epidermis. Ability to stimulate growth and differentiation of keratinocytes connote bioactivity of XD. The natural structure and firmness of XD (composed of collagen and elastic fibres with the surface responding to the basement membrane) enables adhesion,proliferation, migration and differentiation of epidermal cells. In the clinical use XD appertains to temporary covers in burn treatment, particularly on scalds in children. In the frame of grant project IGA there has been investigated healing of widely meshed...
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Electrical Trauma
Werner, Petr ; Königová, Radana (advisor)
While energy flash terrified humanity for thousands of years of history, electricity produced by man serve humanity but also threatening , since 1879 , when Thomas Alva Edison discovered the electric light . its system delivery and use of energy was direct current , which is not suitable for high voltage power transmission over long distance. In 1885, he demonstrated another discovery . George Westinghouse has developed a system using alternating current transformers, which are designed to increase the voltage on the one hand and transmission distribution of energy, and reduce stress on energy consumption. ten years lasted feud between advocates of direct and alternating current, which was ended up using alternating current to Niagara Falls in 1895 and for execution by electric chair in 1900. Passing a current through each wire heats up. electrical work made to the current passes through a conductor , all converted into heat. losses thermal energy is proportional to the resistance wire and increases the second square of the current . Therefore, it is advantageous when high energy performance over long distances transmit energy high voltage at low current in the line. With increasing demands on energy was necessary to develop appropriate preventive measures and technical security, as well as improved methods...
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