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Analysis of male sex pheromone of different population of tropic fruit fly Ceratitis capitata (Diptera, Tephritidae)
Ježková, Zuzana ; Bosáková, Zuzana (advisor) ; Jirošová, Anna (referee)
The Ceratitis capitata is a very important agricultural pest, whose reproduction behaviour is controled by chemical signals. Males initiate mating by creating leks, where they release sexual pheromones to attract females. The main goal of this diploma thesis was to determine the influence of host plants on the composition of male sex-pheromones C. capitata and to compare emanations of wild males with those originating from laboratory population. We studied the chemical composition of volatiles, released by calling males C. capitata from laboratory and two wild populations, using two-dimensional gas chromatography with time-of-flight mass spectrometric detection (GC×GC-TOFMS), gas chromatography with electroantennographic and flame ionization detection (GC-EAD-FID). All data were statistically analyzed by multivariate data analyses. Significant differences were observed in the quantitative and qualitative composition of the chemical emanations among males from the three populations. The GC-EAD-FID analyses revealed fourteen antenally active compounds with a possible behavioral function. Isomenthone, geraniol, bornyl acetate, geranyl acetone and ethyl octanoate were newly identified antenally active compounds of C. capitata male sex pheromone. Statistical analyses indicated that males and females of...
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Description of chemical diversity of polycyclic diterpenes in tropical termites
Horáček, Natan ; Bosáková, Zuzana (advisor) ; Jirošová, Anna (referee)
Ability to defend their nests belongs among the characteristics shared by different groups of social insects, which is often manifested by chemical defense using various secondary metabolites. Structural richness of defensive chemicals undoubtedly reaches its maximum in termites, whose soldiers are known to produce multiple hundred different compounds. These range from linear β- unsaturated aldehydes, through linear terpenoids and cyclic monoterpenes to polycyclic diterpenes with a wide array of oxygenated modifications. These polycyclic diterpenes are characteristic of the evolutionary modern and the most diversified termite subfamily Nasutitermitinae. Diverse species of Nasutitermitinae were extensively studied in the past with respect to the fascinating diversity of new polycyclic diterpenes, with roughly 70 structures being described so far. Yet, this lineage still remains an interesting target of investigations, especially thanks to several genera belonging to Subulitermes group, which were largely ignored by chemical and systematical studies due to their poorly known taxonomy and the small size of their bodies and nests. In my thesis, I focus on South-American representatives of the Subulitermes group, in an attempt to take advantage of modern technical solutions of chemical analytics in...
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Analysis of male sex pheromone of different population of tropic fruit fly Ceratitis capitata (Diptera, Tephritidae)
Ježková, Zuzana ; Bosáková, Zuzana (advisor) ; Jirošová, Anna (referee)
The Ceratitis capitata is a very important agricultural pest, whose reproduction behaviour is controled by chemical signals. Males initiate mating by creating leks, where they release sexual pheromones to attract females. The main goal of this diploma thesis was to determine the influence of host plants on the composition of male sex-pheromones C. capitata and to compare emanations of wild males with those originating from laboratory population. We studied the chemical composition of volatiles, released by calling males C. capitata from laboratory and two wild populations, using two-dimensional gas chromatography with time-of-flight mass spectrometric detection (GC×GC-TOFMS), gas chromatography with electroantennographic and flame ionization detection (GC-EAD-FID). All data were statistically analyzed by multivariate data analyses. Significant differences were observed in the quantitative and qualitative composition of the chemical emanations among males from the three populations. The GC-EAD-FID analyses revealed fourteen antenally active compounds with a possible behavioral function. Isomenthone, geraniol, bornyl acetate, geranyl acetone and ethyl octanoate were newly identified antenally active compounds of C. capitata male sex pheromone. Statistical analyses indicated that males and females of...
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