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Stanovení antibiotické resistence u střevních bakterií selat krmených ZnO a nanočásticemi zinku
Janošťáková, Barbora
The present thesis investigated the antibiotic resistance of gut microbiota in piglets that have been fed supplements of zinc oxide (ZnO) and two types of zinc nanoparticles (ZnA and ZnC). First, an overview has been compiled to elucidate the emergence and spread of antibiotic resistance, possible remedies and their ecologic impact. Zinc nanoparticles have been proven to be more environmentally friendly and therefore a perspective replacement of zinc oxide, which is soon to be removed from use in prevention. This aim of this thesis was to evaluate their ability to prevent antibiotic resistance. To do so, an experiment has been conducted using gut Escherichia coli isolate samples obtained from 100 weaned piglets. The piglets have been randomly sorted into groups of 10 and fed a zinc supplement of set concentration: the control group received no supplementation, the rest of the groups received either ZnO, ZnA or ZnC in a concentration of 500, 1000 or 2000 mg per kilogram of compound feed. The piglets were supplemented for 10 consecutive days, their gut microbiota samples have been collected throughout the duration of the experiment and analysed for antibiotic resistance. Nanoparticles ZnC proved to be a sound alternative to ZnO, having significantly reduced the resistance to most studied antibiotics. The most effective of ZnC group dosing appeared to be 1000 mg/kg of compound feed.
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Comparison of salinity tolerance of selected hemp genotypes grown in vitro
Janošťáková, Barbora
Salinity stress is a threat for present population, but most importantly for future generations. Salinization of soil is a current issue, which should not be underestimated. Most of the usual crops are not physiologically adapted to survive in saline soil, which means reduced yields of edible plants. As the population grows and the harvest decreases, it is becoming clear that the ratio is getting imbalanced and there is a serious threat of food shortage. This thesis aims to assess and follow the negative effects of NaCl on hemp plants which have been cultivated in vitro. The impact of NaCl was monitored and recorded. The results were shown in graphs, which helped to evaluate more clearly the effect of NaCl on hemp plants. NaCl affected morphology and specific physiological parameters of Beniko and Fibrol hemp breed. The results clearly show the influence of increasing NaCl concentration on plants including changes at the cellular level.
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