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Využití mykorhizy při vegetativním množení dřevin bylinnými řízky
Ján, Jakub
The topic of the diploma thesis was the study of the influence of commercial mycorrhizal preparation on the rooting of softwood cuttings of two selected species of ornamental shrubs (Forsythia × intermedia 'Maluch' and Weigela florida 'Piccolo'). The main objective was to find out whether the mycorrhizal preparation can make the process of vegetative propagation more efficient and favourably influence the formation of roots. The used preparation contained arbuscular mycorrhizal fungi of the genus Glomus, two different amounts of the preparation were used. The experiment was carried out in the summer months of the year 2016. The experiment was founded in a plastic greenhouse on the ground of the Faculty of Horticulture in Lednice in Moravia (the Czech Republic), two main terms of propagation passed (in June and August) and one supplementary term passed (in August). The total number of adventitious roots, the number of branched roots, the length of shoots and the mortality of cuttings were evaluated. Mycorrhizal colonization of roots was evaluated in the laboratory under the microscope. The results of the experiment did not show that the used mycorrhizal preparation can positively affect the rooting of softwood cuttings, the application of the mycorrhizal inoculum did not improve the efficiency of vegetative propagation and did not provide convincing advantages. Mycorrhizal fungi did not stimulate the growth of cuttings, and in fact they did not influence the number of adventitious roots neither in Forsythia × intermedia 'Maluch', nor in Weigela florida 'Piccolo'. The only significant positive was that the Weigela florida 'Piccolo' had a higher number of branched roots after inoculation. The observation of roots under the microscope showed that the roots of both species were colonized by arbuscular mycorrhizal fungi, but the extent of colonization did not reach high levels. Mycorrhizal inoculation of propagation substrate cannot be recommended as an effective method that would improve rooting. However, it should be added that the experiment only passed for one year and the results were based only on one term of propagation. Thus the influence of mycorrhizal fungi on the rooting of plant cuttings should be the subject of further research.
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