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Comparison of basic coagulation methods - APTT, PT, FBG by mechanical and optical principle
Hricová, Júlia ; Fátorová, Ilona (advisor) ; Sadílek, Petr (referee)
In my thesis, the basic coagulation methods APTT, PT and fibrinogen were compared with the use of an optical and impedance principle. The coagulation analyser Sysmex CA 7000 was used for measuring coagulation tests using an optical method and the coagulation analyser AMAX Destiny Plus measured the formation of clots using an impedance method. In a theoretical part of my thesis, I described the process of formation of clots as well as the detection principles of the two analysers mentioned. I mainly focused on possible interferences which can influence the values of coagulation tests. I described how these interferences could develop, the mechanism of their influence on measured parameters and how their effects could be removed. In a practical part of my thesis, I described a preanalytic part of blood taking, the coagulation analysers and a principle of their measurement, an internal quality control and the reference values for these tests. For the coagulation tests APTT, PT and fibrinogen were used patient's normal, haemolytic, lipemic and icteric plasma. T-test done in Microsoft Excel was used to assess similarities or differences between the optical and measured impedance results. To see the differences between methods, graphs of mean values of each method were created. For the conclusion of my...
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Comparison of basic coagulation methods - APTT, PT, FBG by mechanical and optical principle
Hricová, Júlia ; Fátorová, Ilona (advisor) ; Sadílek, Petr (referee)
In my thesis, the basic coagulation methods APTT, PT and fibrinogen were compared with the use of an optical and impedance principle. The coagulation analyser Sysmex CA 7000 was used for measuring coagulation tests using an optical method and the coagulation analyser AMAX Destiny Plus measured the formation of clots using an impedance method. In a theoretical part of my thesis, I described the process of formation of clots as well as the detection principles of the two analysers mentioned. I mainly focused on possible interferences which can influence the values of coagulation tests. I described how these interferences could develop, the mechanism of their influence on measured parameters and how their effects could be removed. In a practical part of my thesis, I described a preanalytic part of blood taking, the coagulation analysers and a principle of their measurement, an internal quality control and the reference values for these tests. For the coagulation tests APTT, PT and fibrinogen were used patient's normal, haemolytic, lipemic and icteric plasma. T-test done in Microsoft Excel was used to assess similarities or differences between the optical and measured impedance results. To see the differences between methods, graphs of mean values of each method were created. For the conclusion of my...
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