|
|
Sledování antioxidační aktivity u laboratorního potkana při zkrmování barevné pšenice Konini
Holeksová, Veronika
In the experiment, we observed how it affects feeding colored wheat grains with purple color on the antioxidant activity in the liver and in the blood of rats. For the experiment, we used 64 male rats Wistar albino four to six weeks. The experiment was performed four weeks. Experimental group (n=32) rats fed the only color wheat Konini. Purple wheat Konini (14.01 mg/g of anthocyanins). The control group (n=32) are fed conventional wheat Bohemia mixed with gluten to supplement the crude protein content as Konina (16%). During the experiment, we looked at three day intervals additions and weighed daily feed consumption. Antioxidant activity was measured by methods DPPH, FR, FRAP and ABTS. Results of measurement of antioxidant activity in the liver appeared inconclusive for all the methods. Only the results of the FR and FRAP with blood appeared conclusive. At control FR mg/l 297,35+-23,94 and Konini 215,57+-12.16 .FRAP mg/l control 28,78+-1,35 and 23.86 +-1.28 Konin. Other methods for blood came out inconclusive as well as in the liver. We found that liver weight in rats fed wheat Konin was 7% lower than in control rats. The results were processed using a statistical method ANOVA Scheffe test.
|
guest ::
