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Separation of molecular probes by capillary electrophoresis
Hof, Maxim ; Kučera, Radim (advisor) ; Lochman, Lukáš (referee)
Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy in Hradec Králové, Charles University Candidate: Maxim Hof Supervisor: doc. PharmDr. Radim Kučera, Ph.D. Consultant: Mgr. Ondřej Horáček Title of Thesis: Separation of molecular probes by capillary electrophoresis Oligonucleotides are organic molecules which find their application in fields such as biotechnology, molecular biology, diagnosis and therapy. For the use of oligonucleotides (ON's) as molecular probes, it is necessary to covalently modify the ON's for example with a fluorescent dye and a quenching moiety. The products of this labelling reactions are the required double labelled ON probe, but also side products like the unlabeled ON and the ON's labeled either with the fluorescent dye or the quencher. Consequently, the products of this reaction must be separated, which represents the focus of this diploma thesis. The separation of these double labeled ON probes has been so far only performed by chromatographic approaches. The aim of this thesis is to explore the use of capillary electrophoresis for that separation. Specifically, the use of capillary electrophoresis was investigated for the separation of a) single-labeled ON probe with BHQ quencher from a double-labeled ON probe with a BHQ quencher and...
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