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Supercritical fluids for extraction of biologically active compounds from Eucalyptus leaves
Herbsová, Diana ; Pilařová, Veronika (advisor) ; Sklenářová, Hana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Diana Herbsová Supervisor: PharmDr. Veronika Pilařová, PhD. Title of the diploma thesis: Supercritical fluids for extraction of biologically active compounds from Eucalyptus leaves Eucalyptus is nowadays used for its anti-inflammatory, antibacterial, and antioxidant effects in the pharmaceutical and cosmetic industries. Eucalyptus contains various compounds including flavonoids, volatile compounds, terpenoid acids, and phenolic acids. The aim of this work was to develop a two-step selective extraction method for the extraction of compounds of different physicochemical properties from Corymbia citriodora leaves using extraction solvent containing CO2. Supercritical CO2 is a non-polar solvent with polarity and physicochemical properties, that can be changed by mixing with a suitable polar solvent. In this way, a wide range of compounds with different polarities can be extracted. In this study, the key parameters, including temperature in the range of 40-80 řC, pressure in the range of 100-300 bar were optimized using Box-Behnken design with heptane and ethanol/H2O as a cosolvent. After optimalization of the key parametres, the extraction kinetics were tested at flow rates of 2 mL/min and 4...
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