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Detekce a kvantifikace trinitrotoluenu v reálných vzorcích s použitím metody ELISA
 Hanusová, Lucia ; Chocholoušová Havlíková, Lucie (advisor) ; Chocholouš, Petr (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of: Analytical chemistry Candidate: Lucia Hanusová Supervisor in Hradec Králové: PharmDr. Lucie Havlíková Ph.D Supervisor in Bologna: Prof. Steffano Girotti Title of Diploma Thesis: Detection and quantification of trinitritoluene in real samples by using ELISA method. The 2,4,6-trinitrotoluene (TNT) is a widely used explosive. TNT has been used extensively in the manufacture of explosives since the beginning of last century. It could be found as a contaminant in environment, contaminates air, water and soil. It is accumulated in plants and fishes, and could affect human health. A reliable and sensitive method is needed to get the information about pollution in environment, to detect exposion in human organism or to identify the explosive after explosion. A new rapid method of indirect competitive Enzyme-Linked ImmunoSorbent Assay method with chemiluminescent detection (CL-ELISA) for determination of trinitrotoluene in real samples was successfully applied. The immunological analysis using many antibodies was performed after extraction to methanol (MeOH). The antibody labelled with horse-radish peroxidase was added to reaction and after combination with luminescent mixture the light was developed and we were able to...
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