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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Proteome dynamics during seed germination
 Habánová, Hana
Seed germination is a crucial phase of the plant life cycle. Despite the huge progress that has been made in the last decade, the molecular mechanisms regulating seed germination are far from being resolved. Most of the early events of germination are mediated by molecules stored in seeds during the seed development and thus we employed proteomic analyses to elucidate the mechanism behind this process. This thesis - Proteome dynamics during seed germination - reviews present-day knowledge of this topic. In the experimental part, the results of three related research projects are summarized. First, the effects of a novel class of growth regulators were analyzed in the barley germination assay and the optimal concentrations were determined. The methodology for an improved analysis of barley seed proteins was developed and the optimized targeted analysis will allows the detection of over 4,000 proteins. Finally, Arabidopsis thaliana seed proteome was analyzed and the results indicate that (i) an alternative MS/MS data processing significantly improves the detection limits and (ii) the proteasome-ubiquitin system plays a crucial role in adjusting the seed proteome dynamics during the germination progress.
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Vliv růstových regulátorů na proteom rostlin v průběhu klíčení
 Habánová, Hana
Seed germination is a complex process during which an embryonic plant contained within a seed transforms into a seedling. The majority of species, including model plant Arabidopsis thaliana produce dry seeds that will fully re-establish metabolism after imbibition. This early phase of a plant's life is highly important for its survival and its progress depends on both internal and external stimuli. This thesis entitled "Effects of plant growth regulators on proteome dynamics during seed germination" is focused on effects of light and plant growth regulators on germination. To get an insight into molecular mechanism of germination, proteome dynamics in germinating Arabidopsis were investigated. Seeds were imbibed in presence of plant growth regulators in continuous light or dark and material for LC-MS proteome profiling was harvested after 24 and 48 hours. Altogether, abundances of more than 1100 proteins were followed. PCA analysis and a detailed pair-wise comparison of mock-treated seeds provided evidence that the modern proteome-wide analysis is a promising tool for plant germination research.
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