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Zavedení helminta jako modelového organismu do kultury a charakterizace imunitní odpovědi hostitele
 HLOŽKOVÁ, Oldřiška
The main goals of the present thesis were (i) to introduce a model commensal tapeworm, Hymenolepis diminuta, to the culture and (ii) characterization of a mucosal immune response in the host gut. Primarily, I focused on the establishment of a culture of H. diminuta under laboratory conditions based on its natural life cycle - grain beetles (Tenebrio molitor) as an intermediate host and outbred Wistar rats as definitive hosts. Infected beetles were bred at 28°C and due to this specific condition we were able to get infectious larvae three weeks after the first infection. To meet the second aim of this study, I examined a production of IgA antibodies against H. diminuta in the intestine of definitive host in the fecal samples using an indirect ELISA method. First, I performed several experiments focused on the optimization of H. diminuta antigen suitable for detection of IgA antibodies against H. diminuta in ELISA. Subsequently, I performed two experiments for detection of IgA fluctuation during H. diminuta infection in the rat model system. The strong production of IgA antibodies started always in the patent period of infection (i.e., when adult tapeworm is developed). In summary, my results suggest that H. diminuta has a strong immunomodulatory effect on the host organism.
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