Home > Search Results: HAVRAN, Jiří
Search:
Search Tips :: Advanced Search
Search collections:
Sort by: Display results: Output format:
National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Detection of Lyme disease spirochetes in clinical samples by PCR-based methods and optimalization of conditions borrelia cultivation conditions from samples of patients with LB symptoms
 HAVRAN, Jiří
The samples under investigation were collected in Department of Pediatric Infectious Diseases of the University Hospital (Brno). Group of patients (100) was heterogeneous in terms of symptoms, age and sex. The samples were taken from patients with an LB diagnosis and from those with nonspecific symptoms. Molecular typing of LB spirochetes in clinical samples (104 blood/serum, 89 cerebro-spinal fluid and 1 synovial fluid) became necessary when the general immunological tests gave unclear results. The samples were analyzed using PCR-based and molecular biology techniques that include: nested- and spacer-PCR, specie-specific PCR, sequence, virtual hybridization, in silico RFLP analysis, similarity search. Results of conducted analysis confirmed that 51% of samples (98) were positive on B. bugrdorferi sensu lato. Using above mentioned techniques 6 spirochete species from B. burgdorferi sensu lato complex were identified; two of them weren?t detected in samples of human origin in Europe yet. Comparative analysis of two media for Borrelia cultivation from samples of human origin definitely proved the adventage of using MKP instead of traditionally used BSK-H Complete.
Detailed record
Identification and isolation gene encoding peroxiredoxin, detoxification enzyme involved in antioxidant defense of hard tick Ixodes ricinus.
 HAVRAN, Jiří
Antioxidant enzymes play an important role in protection against the oxygen radicals generating during aerobic metabolism, as well as in defense against host immune responses. Peroxiredoxin isolated from the hard tick Ixodes ricinus belongs to 1CysPrx group of peroxiredoxins. It is expressed in all stages of hard tick (larva, nymph and adult) and in all tissues examined. The full gene (EU373821) is 756 nt long and consists of 251 amino acids, encoding 27.94 kDa protein. This is the first report of isolation of detoxification enzyme (peroxiredoxin) involved in antioxidant defense of hard tick Ixodes ricinus.
Detailed record

See also: similar author names
4 Havran, Jan
2 Havran, Josef
Interested in being notified about new results for this query?
Subscribe to the RSS feed.
Digital Repository :: Search :: Submit :: Personalize :: Help
Powered by Invenio v1.1.2
Maintained by admin.invenio@techlib.cz
This site is also available in the following languages:
Česky  English