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Comparative analysis of RNA binding properties of Rpl22 proteins
Gredová, Alexandra ; Abrhámová, Kateřina (advisor) ; Vopálenský, Václav (referee)
After the whole genome duplication event, Saccharomyces cerevisiae lost 90 % of its paralogs. 59 ribosomal protein genes (RPG) retained a duplicated form. The cell has to balance expression of RPGs as a part of adaptation to changing conditions, thus ensuring the production of the right ratio of ribosomal proteins (RP). In addition, RPGs contain 1/3 of all introns found in the S. cerevisiae genome. Rpl22A/B are part of the large ribosomal subunit where they contact 25S rRNA. Deletion of these RPGs results in 2X slower growth in comparison with WT cells. Within their extraribosomal function Rpl22A/B are able to intragenically and intergenically regulate their expression. Binding of Rpl22A/B to the intronic part of pre-mRNA of RPL22A or RPL22B results in splicing inhibition, which is stronger in the case of the RPL22B intron (RPL22Bi). However, the exact mechanism is not known. We know that Rpl22s from various organisms bind a hairpin structure that can be found in 25S rRNA also. Since the predicted structure of RPL22Bi does not contain this binding motif, it rises a question of whether the RNA binding surface of Rpl22A/B is different or more extensive than the one with which Rpl22 contacts 25S rRNA. We compared the ability of Rpl22 from different organisms to complement the functions of Rpl22A/B. These...
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Extraribosomal function of ribosomal proteins
Gredová, Alexandra ; Folk, Petr (advisor) ; Roučová, Kristina (referee)
Ribosomal proteins are important not only because they enable proteosynthesis, but also because of their functions outside of ribosome - in their extraribosomal functions. This thesis is mainly focused on the extraribosomal functions of higher eukaryotic ribosomal proteins. Specifically, it analyzes three ribosomal proteins, L13a, L22, and S3, which illustrate several aspects of extraribosomal functions. It discusses the mechanisms of action of these proteins and how they affect various levels of gene expression. Key words: extraribosomal function, L13a, L22, S3, p53, NF - κB, splicing
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