|
|
HPLC study of the role of pyridoxal isonikotinoyl hydrazone in isoniazid intoxication
Gladziszová, Marcela ; Kovaříková, Petra (advisor) ; Kučera, Radim (referee)
High-performance liquid chromatography belongs nowadays among the most frequently used analytical methods in all fields of the pharmaceutical analysis. It makes both qualitative and quantitative evaluation possible. Isoniazid is used for the treatment and prevention of tuberculosis. One of the most serious symptoms of toxicity is a neurotoxicity which is connected with the B6 deficiency. The neurotoxicity probably relates to the formation of condenzation products isoniazid and vitamins B6 - such as pyridoxal isonikotinoyl hydrazone (PIH). Concrete evidence of the ongoing mechanisms and actions concerning this problemsis remains still elusive. The aim of this thesis was the optimalisation of the chromatography conditions for the separation of 8 analytes (isoniazid, acetylisoniazid, pyridoxal, pyridoxol, pyridoxamine, pyridoxal-5-phosphate, PIH a the internal standard o- 108). Therafter, the isolation of these compounds from plasma samples using precipiation was optimized. The separation was performed on a LiChroCART 250 × 4 mm I.D., analytical column with Lichrospher 100 RP-18, 5 µm packing, protected with a Purospher RP-18, 5µm guard column. As the mobile phase A was used aqueous solution of NaH2PO4 (0,01 mol/l), with the addition of EDTA (0,001 mol/l) and heptansulphonic acid (0,005 mol/l) with...
|
|
|
HPLC study of the role of pyridoxal isonikotinoyl hydrazone in isoniazid intoxication
Gladziszová, Marcela ; Kovaříková, Petra (advisor) ; Kučera, Radim (referee)
High-performance liquid chromatography belongs nowadays among the most frequently used analytical methods in all fields of the pharmaceutical analysis. It makes both qualitative and quantitative evaluation possible. Isoniazid is used for the treatment and prevention of tuberculosis. One of the most serious symptoms of toxicity is a neurotoxicity which is connected with the B6 deficiency. The neurotoxicity probably relates to the formation of condenzation products isoniazid and vitamins B6 - such as pyridoxal isonikotinoyl hydrazone (PIH). Concrete evidence of the ongoing mechanisms and actions concerning this problemsis remains still elusive. The aim of this thesis was the optimalisation of the chromatography conditions for the separation of 8 analytes (isoniazid, acetylisoniazid, pyridoxal, pyridoxol, pyridoxamine, pyridoxal-5-phosphate, PIH a the internal standard o- 108). Therafter, the isolation of these compounds from plasma samples using precipiation was optimized. The separation was performed on a LiChroCART 250 × 4 mm I.D., analytical column with Lichrospher 100 RP-18, 5 µm packing, protected with a Purospher RP-18, 5µm guard column. As the mobile phase A was used aqueous solution of NaH2PO4 (0,01 mol/l), with the addition of EDTA (0,001 mol/l) and heptansulphonic acid (0,005 mol/l) with...
|
guest ::
