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Separation of pharmaceutically active compounds by mixed-mode and chiral chromatography: interaction study
Fojtíková, Sofia ; Bosáková, Zuzana (advisor) ; Kozlík, Petr (referee) ; Fischer, Jan (referee)
During the drug development process, the pharmaceutical industry often relies on chromatographic techniques. High-performance liquid chromatography (HPLC) is among the most commonly used chromatographic techniques for these purposes, with reverse phase and HILIC being the most common choices. However, despite their popularity, these techniques have several drawbacks, such as the contribution of secondary interactions that arise from the nature of the carrier material, and limitations in terms of analyzing molecules with certain properties. In this sense, multimodal (also called mixed-mode) chromatography (MMC) appears to be a promising technique capable of addressing these challenges. The first part of the dissertation is focused on the comparison of the retention of structurally different molecules in different chromatographic systems using seven different multimodal stationary phases and the effort to elucidate the interaction mechanisms applied to the individual studied stationary phases. By observing the trends, obtained under different conditions for molecules with different physicochemical properties, it was at least partially achieved. Chiral chromatography is another key tool in the analysis of pharmaceuticals. Its main purpose in the development of medicinal products is to separate...
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