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Development of novel approaches to automated sample preparation for pharmaceutical and environmental analysis
Fikarová, Kateřina ; Sklenářová, Hana (advisor) ; Macka, Miroslav (referee) ; Polášek, Miroslav (referee)
Institution: Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Kateřina Fikarová Supervisor: Assoc. Prof. Hana Sklenářová, Ph.D. Co-supervisor: Burkhard Horstkotte, Ph.D. Title of the Dissertation Thesis: Development of novel approaches to automated sample preparation for pharmaceutical and environmental analysis Since their introduction 60 years ago, flow techniques have become a popular tool for automation of various analytical processes and were applied in environmental, pharmaceutical, or food analysis. Above all, they are recognized for the small consumption of reagents, fast analysis in an automated manner, the possibility to study kinetics of the reactions, and their versatility of the operation. This dissertation contributes to the field of automation of sample preparation and leaching studies of the environmental pollutants using flow techniques, mainly the technique Lab-In-Syringe. The theoretical part gives the reader some insight into the possibilities and advantages of automation, with emphasis to flow techniques. This section is divided into three main chapters with the first one dedicated to automation. The four subchapters are focused mostly on the flow techniques and their general principles, different instrumentation, and...
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Development of novel approaches to automated sample preparation for pharmaceutical and environmental analysis
Fikarová, Kateřina ; Sklenářová, Hana (advisor) ; Macka, Miroslav (referee) ; Polášek, Miroslav (referee)
Institution: Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Kateřina Fikarová Supervisor: Assoc. Prof. Hana Sklenářová, Ph.D. Co-supervisor: Burkhard Horstkotte, Ph.D. Title of the Dissertation Thesis: Development of novel approaches to automated sample preparation for pharmaceutical and environmental analysis Since their introduction 60 years ago, flow techniques have become a popular tool for automation of various analytical processes and were applied in environmental, pharmaceutical, or food analysis. Above all, they are recognized for the small consumption of reagents, fast analysis in an automated manner, the possibility to study kinetics of the reactions, and their versatility of the operation. This dissertation contributes to the field of automation of sample preparation and leaching studies of the environmental pollutants using flow techniques, mainly the technique Lab-In-Syringe. The theoretical part gives the reader some insight into the possibilities and advantages of automation, with emphasis to flow techniques. This section is divided into three main chapters with the first one dedicated to automation. The four subchapters are focused mostly on the flow techniques and their general principles, different instrumentation, and...
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Set-up and optimization of automated in-syringe extraction of trace metals for ICP measurement
Fikarová, Kateřina ; Horstkotte, Burkhard (advisor) ; Solich, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Kateřina Fikarová Supervisor: Burkhard Horstkotte, Ph.D., M.Sc. Consultant: Doc. PharmDr. Hana Sklenářová Ph.D. Work title: Set-up and optimization of automated in-syringe extraction of trace metals for ICP measurement The pollution of the environment by transition metals has to be monitored by sensitive methods due to the possible accumulation in humans and toxicity with respect to human health. For the first time, dispersive liquid-liquid extraction followed by back- extraction using the automation technique Lab-In-Syringe has been studied for the determination of Cd, Cu and Pb by inductive coupled plasma atomic emission spectrometry (ICP-AES). The method was automated using the void of a syringe pump of a sequential injection analysis (SIA) system as extraction vessel. A magnetic stirring bar was used for mixing the syringe's content and to disperse the extraction solvent. A motor holding neodymium magnets was used to force stirring rotation. Controlled by software, stirring could be stopped for phase separation. After setup and adjustment of the system and the operation procedure for automated extraction, preliminary experiments were done to evaluate the appropriate reagents....
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