|
CSL proteins of Schizosaccharomyces pombe
Převorovský, Martin ; Půta, František (advisor) ; Dvořák, Michal (referee) ; Kořínek, Vladimír (referee)
The CSL family of transcription factors is essential for metazoan development,mostly due to their involvement in the Notch signaling pathway. We identified two novel classes of CSL genes in several fungal species, organisms lacking the Notch pathway. We characterized experimentally cbfl I- and cbfl2*, the two CSL genesof Schizosaccharomyces pombe, in order to elucidate the CSL function in fungi. We provide evidence supportingtheir identiý as genuine CSL genes.Both cbfll- and cbfl2- are non-essential; they have distinct expression profiles and code for nuclear proteins with transcription activation potential. Significantly, we demonstratedthat Cbfl1 recognizesspecifically the canonical CSL response element GTGA/6GAA in vitro. The deletion of cbfLt_ is associated with growth phenoýpes and altered colony morphology. Furthermore, we found that Cbfl I and CbfIZ play opposite roles in cell adhesion, nuclear and cell division and their coordination. Disturbed balance of the two CSL proteins leads to cell separationdefects,cut phenotype, and high-frequency diploidization. Our data show that CSL proteins operate in an organism predating the Notch pathway, which should be of relevance to the understandingof (Notch- independent)CSL functions in metazoans.
|
|
Design of Communal Car
Dvořák, Michal ; Jelenčík, Branislav (referee) ; John, David (advisor)
The market for municipal carriers is showing potential with the advent of new technologies, especially in the way of propulsion. These changes may affect the design and concept of the carrier itself. The topic of this diploma thesis is the design of a communal carrier for implements. The aim of the text is to guide the reader through the course and result of the work. the work is the design of a utility carrier for the terrain, using an electric drive. The work deals with technical, ergonomic, shape, color and material solutions. The cabin of the carrier is designed in such a way that it allows a wide view when working, and the appearance of the carrier is, thanks to the minimalistic approach to design, a unique and distinctive character of electromobility.
|
|
New technologies for biometric recognition based on hand characteristics
Dvořák, Michal ; Jadlovský, Ján (referee) ; Marcialis, Gian Luca (referee) ; Drahanský, Martin (advisor)
Biometrie nám umožňuje identifikovat či verifikovat osoby na základě jejich fyzických vlastností. Přestože identifikace samotná je velmi rychlá, extrakce zvolené biometrie v reálném světě je netriviální problém. Pro snímání většího množství lidí, například na letišti, potřebujeme dosáhnout snímání a rozpoznání v co nejkratším čase, ideálně za chůze uživatele. Tato disertační práce se zabývá vývojem bezdotykového a tedy hygienického biometrického systému, který je schopen provést extrakci identifikačních charakteristik ruky a následně rozpoznat uživatele za běhu. Spolu s tím práce obsahuje další systémy a algoritmy potřebné pro řešení tohoto komplexního problému. Práce prezentuje současný stav výzkumu a vývoje v oblasti biometrické identifikace na základě vybraných charakteristik, se zaměřením na identifikaci pomocí geometrie ruky a otisků prstů. Dále se pak v2nuje technologickým principům využitých pro sběr těchto biometrických charakteristik. Dle disertačních cílů, formulovaných na základě rešerše, se praktická část této práce zabývá vývojem biometrických systémů pro použití k identifikaci velkého množství lidí za běhu, pro které většina současných biometrických systémů založených na charakteristikách ruky nejsou vhodné. Dále pak se práce zabývá souvisejícími problémy, zejména pak detekcí prezentačního útoku a biometrickou multimodalitou.
|
|
Traffic Situation Model
Dvořák, Michal ; Petyovský, Petr (referee) ; Horák, Karel (advisor)
In this thesis, parameters required for physical construction of model of traffic camera system are identified. The information is used to design and construct a model, which supports simulation of important traffic situations. With focus, on the ability of model to allow for digital modification of simulation parameters over, for this purpose created, communication interface. The next part of thesis then focuses on creation of application which supports the control and image retrieval from group of cameras. The application is designed to support settings necessary for use as average speed enforcement cameras and as red-light cameras.
|
|
Firearm Type Identification in an Image
Čech, Ondřej ; Drahanský, Martin (referee) ; Dvořák, Michal (advisor)
Main goal of this work is to design, implement and test an approach for classifying firearms in an image into categories with short and long fireams, and then with single shot, multi-barreled, repeating and semi-automatic/automatic firearms. This problem was solved using SVM classifier together with Harris corner detector, FREAK descriptor and Bag of Words method. Accuracy of final program is up to 13,3 %.
|
|
Anti-Drone Perimeter Protection
Janík, Roman ; Dvořák, Michal (referee) ; Drahanský, Martin (advisor)
Developement of drone technology brings opportunities for many fields of human activity, but simultaneously brings security threats. A need to effectively face these threats arises. In this work is described the problematics and state-of-the-art methods for object detection in a video captured by moving camera. A system for detecting and locating a drone or a flock of drones has been proposed. Algorithm for detection is based on convolutional neural network, specifically on SSD algorithm. The convolutional neural network was trained on self-made dataset. The system was implemented using OpenCV library with possible algorithm acceleration on GPU using OpenCL. Created solution was tested on video.
|
|
Virtual Gate for Counting the Passing of Persons
Chudý, Andrej Oliver ; Dvořák, Michal (referee) ; Drahanský, Martin (advisor)
The goal of this thesis is to propose a viable technique of detecting people passing through a virtual line (any opening without a turnstile or other form of the physical obstacle). Two software solutions were implemented to achieve high accuracy. First one processes 2D data output from webcam and the other one processes depth data from the 3D imaging sensor. Both software solutions were deployed and tested in a pharmaceutical storage room, where they achieved the accuracy of 98%. The software proposed in this thesis is thus reliable enough to become the basis of access control and security systems or for real-time evaluation of visitor rate statistics of commercial properties and events. The thesis also contains a comparative analysis of 3 widely used depth sensors.
|
|
Lego Mindstorm EV3 in Education of Programming and Robotics
Páral, Jaroslav ; Dvořák, Michal (referee) ; Orság, Filip (advisor)
In this bachelor thesis, we tackle the problem of software development for the Lego EV3 brick. We want to allow the beginner programmers to create complex programs that cannot be easily created using the original graphical programming interface.We provide comparison of several alternatives of the original system from performance and user point of view. Based on this comparison, we choose EV3RT as base for our work.We also present our object-oriented API built on top of the EV3RT system to ease the transfer from graphical to text programming. Therefore, we provide a suitable platform for the beginners, who already reached the limits of standard system and want to develop more complex programs demanding more computing power.
|
|
Manipulator Control for Acquisition of the Eye Retina
Malaník, Petr ; Dvořák, Michal (referee) ; Drahanský, Martin (advisor)
To use the image of the retina for biometric or medical purposes, it is necessary that the image is of the highest quality and ideally covers the largest possible area. It is therefore necessary to move the sensing device to the most suitable position while ensuring its stability. A laboratory handling platform is used for this purpose. The platform is moved by stepper motors. A control board equipped with a microcontroller has been developed for their control, which allows very fine movements. The theoretical accuracy of the manipulator is up to 20 nm. Since it is necessary for sensing, to have proper retina illumination, the control system also includes adjustable illumination elements in the infrared and visible spectrum. The resulting system allows scanning of the retina of the eye from multiple angles and thereby effectively increase the area on which it is possible to further look for diseases or biometric features.
|
|
Association Rules Mining
Dvořák, Michal ; Chmelař, Petr (referee) ; Stryka, Lukáš (advisor)
The main goal of this bachelor's thesis is design and implementation of the application that provides a comparison of the performance and time consumption of given algorithms for mining of the frequent itemsets and the association rules. For demonstration, the mining algorithms Apriori, AprioriTIDList, AprioriItemSet and the method using FP-tree were chosen. The tests were executed over various amounts of data and with different minimum support and confidence values as well. The application was implemented in the object oriented language C# and the relational database provided by MS SQL Server 2008 is used as the data source.
|