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Comparison of the microbial composition of English Blue Stilton cheese with related cheeses
Druláková, Tereza ; Vodička, Juraj (referee) ; Brázda, Václav (advisor)
This bachelor’s thesis focuses on the comparison of English blue-veined cheese Blue Stilton with commonly available cheeses of the same type, Roquefort and Niva, in terms of microbial composition, aromatic volatile content and also by sensory analysis. These methods distinguish the expensive Blue Stilton cheese from common and cheaper cheeses of the same type in the Czech Republic. The presence of 15 selected species of microorganisms was determined by RT-PCR. Three of them proved to be specific for Blue Stilton, specifically bacteria Brevibacterium linens and the yeasts Geotrichum candidum and Kluyveromyces lactis. Aromatic volatile compounds were determined by HS-SPME-GC-MS. Heptan-2-one, nonan-2-one, non-8-en-2-one and hexanoic and octanoic acids were the most important substances contributing to the aromatic profile of all cheeses. The cheeses can be distinguished according to the content of these substances in their aromatic profile or according to the amount of these substances in the individual samples. The sensory evaluation revealed that in most parameters like appearance, consistency and taste, Niva cheese was the most acceptable for Czech consumers while Blue Stilton was the most popular in the aroma category. On the basis of the obtained data, we are able to distinguish English Blue Stilton from other blue-veined cheeses according to specific microorganisms and the unique aromatic profile.
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