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The role of SGIP1 protein interaction with cannabinoid receptor 1 in nociception under pathological conditions.
Dresslerová, Denisa ; Špicarová, Diana (advisor) ; Zímová, Lucie (referee)
The cannabinoid receptor type 1 (CB1) is a component of the endocannabinoid system and is abundantly expressed in the central nervous system. CB1 receptor has been extensively studied primarily due to its inhibitory role in neurotransmitter release from presynaptic terminals, and this process subsequently modulates postsynaptic currents and postsynaptic neuron excitation. Its interaction with the Src homology domain 3 growth factor 2 receptor binding protein (SGIP1) has recently been investigated in the context of nociception modulation (Hájková et al., 2016b). However, the role of this CB1 regulatory protein, SGIP1, in pathological pain conditions has not yet been described. Clarification of the role of SGIP1 interaction with CB1 receptor may help future research based on the use of cannabinoids as potential therapeutics. The thesis is structured into two parts, theoretical and practical. In the theoretical part of the thesis, knowledge of the given topic is summarized. First, pain is described from an anatomical-physiological perspective, followed by a description of the endocannabinoid system, where a part is dedicated to the CB1 cannabinoid receptor itself, and then another part is dedicated to the SGIP1 protein and its interaction with the CB1 receptor. In the practical part, the role of SGIP1...
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The role of cannabinoid receptor 1 in the modulation of nociceptive signaling at spinal cord level
Dresslerová, Denisa ; Špicarová, Diana (advisor) ; Kořínek, Miloslav (referee)
Pain is an integral part of our lives, it warns of possible impending tissue damage and represents a form of protection for the body. Pain can become directly harmful if it persists. It is perceived individually in different intensity, place and length. In most cases, it is a very unpleasant and robust emotional experience. Before pain perception, nociceptive signalling occurs. When a harmful stimulus is detected in peripheral tissues, free nerve endings are activated, the plasma membrane is depolarized and generated action potential convoys to information about the presence of a nociceptive stimulus to the dorsal horn of the spinal cord. The process of nociception includes stimulation of nerve endings, transduction, signal transmission by nerve fibers into the spinal cord, synaptic transmission and conducting an action potential to the brain, where nociceptive signaling is evaluated. An important area for nociceptive modulation is the dorsal horn of the spinal cord, where synaptic transmission occurs between primary and secondary nociceptive neurons. The individual components of the endocannabinoid system play a significant role in the modulation of synaptic transmission. The major cannabinoid receptors are cannabinoid receptors 1 (CB1) and 2 (CB2), and many endocannabinoids also activate...
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