|
|
název v anglickém jazyce není uveden
Kleiblová, Petra ; Calda, Pavel (advisor) ; Dostálová, Ivana (referee) ; Čechák, Petr (referee)
23 7 SUMMARY This work deals with the role of adipose tissue-derived hormones - adipokines in the development of insulin resistance in pregnant women with gestational diabetes mellitus (GDM). The study consists of two parts. In the first one, the characterization of changes in concentrations of circulating adiponectin - the most abundant adipokine - during menstrual cycle in healthy women was performed. The second part was focused on the changes of gene expression in pregnant women with GDM relative to healthy pregnant controls (14 vs. 13 subjects). Specificial attention was focused on adipokines and their receptors (ADIPOQ, ADIPOR1, ADIPOR2, LEP, RETN, TNF , CFD a PAI1) as well as other genes potentially contributing to the etiopathogenesis of GDM in the samples of placenta, subcutaneous, and visceral adipose tissues obtained during surgical delivery. The changes of gene expression were assessed by the two approaches: Firstly, the gene expression of insulin signaling pathway regulators was characterized using expression arrays in a limited subset of samples from GDM and control subjects. Secondly, the expression of genes that differed most significantly in GDM vs. control group in arrays, together with other genes of involved in the regulation of insulin sensitivity, were analyzed using qPCR in the entire...
|
|
|
název v anglickém jazyce není uveden
Housa, Daniel ; Vernerová, Zdeňka (advisor) ; Dostálová, Ivana (referee) ; Ehrmann, Jiří (referee)
Adipocytokines are a group of adipose tissue-derived cytokines that have been discovered since early nineties. The role of selected adipocytokines in the prostate carcinoma and in selected joint diseases was evaluated. In the first part of this work the role of adiponectin and resistin in the prostate cancer development and progression was examined. Immunohistochemical expression and serum levels of these adipocytokines were evaluated in both groups of patients with benign prostate hyperplasia and prostate cancer of pathological grade pT2 and pT3, respectively. Moreover, selected metabolic, biochemical and anthropometric parameters were included into this study. Serum adiponectin levels did not differ between prostate benign hyperplasia and prostate cancer generally, but they were significantly higher in pT3 in comparison to pT2 group. In agreement with the results of serum levels, tissue immunohistochemistry showed enhanced staining intensity of adiponectin in neoplastic glands and to a lesser degree in prostatic intraepithelial neoplasia while within benign prostate hyperplasia the immunohistochemical staining was generally low. None relation of adiponectin expression to disease grade or stage was observed. In resistin no difference between benign hyperplasia and prostate cancer in respect to serum levels...
|
guest ::
RSS feed.