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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Comparative phenotypic study of selected Arabidopsis formin mutants
 D'Agostino, Viktoria ; Cvrčková, Fatima (advisor) ; Tylová, Edita (referee)
Actin filaments and microtubules are involved in cell development and morphogenesis. Plant Class II formins regulate both cytoskeletal polymers. However their function has not yet been fully described. This study examines effects of LOF mutations in Arabidopsis thaliana FH13 (AT5G58160) and FH14 (AT1G31810) genes on early root system development using a pharmacological approach. Since measuring root length of numerous mutant lines in multiple conditions is laborious and time consuming, this thesis also involves optimization of this process with the aim to establish a reliable method of fast visualisation and measurement of Arabidopsis seedlings in a time series in the laboratory. Furthermore, statistical analysis for a large amount of data gathered in multiple conditions had to be optimized. While no significant phenotype in terms of root length was found in fh13, fh14 and double fh13 fh14 LOF mutants under standard conditions, treatment with cytoskeletal drugs revealed possible changes in lateral root branching in an fh14 mutant. Nevertheless, specific function of FH13 and FH14 remains a question.
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Molecular mechanisms of cell polarity and morphogenesis in moss Physcomitrella patens
 D'Agostino, Viktoria ; Žárský, Viktor (advisor) ; Soukup, Aleš (referee)
Plant cells are able to establish polarity and expand by tip growth. Polarized cells often embrace functions important for plant viability. The process of tip growth requires actin cytoskeleton in collaboration with a number of accessory proteins. The position of the intensively expanding region is provided by microtubules and the function of signalling proteins. Polarized secretion regulates the structural properties and subsequently the shape of the cell wall. Some components of the secretory and signalling pathways are highly conserved among eukaryotes, others are found exclusively in the plant kingdom. Though much has been discovered in yeast and animal cells, many mechanisms in plants are yet to be revealed. Model systems performing tip growth, such as root hairs, pollen tubes and protonema cells, enable comparison and thus a complementary overview of the various processes.
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