|
|
Vliv eikosapentaénové a dokosahexaénové kyseliny na expresi vybraných genů podílejících se na modulaci zánětlivé reakce u modelového organismu
Charousová, Markéta
In my study thesis Effect of eikosapentaenoic and dokosahexaenoic acids on expression of selected genes, which participate in modulation of inflammatory reaction at model organism I evaluated expression of genes GPR120, PPARgamma, LBP, ICAM, Il-4, Il-10, Il-1beta and TGF-beta. As model organism was selected pig. The pigs were divided in two groups, the control group was fed with 2,5% addition of palmic oil (P), the second group was fed with 2,5% addition of fish oil. After 70 days long fattening was each group divided into halfs. One half of each group was stimulated LPS (P+ and R+). Liver and adipose tissue were collected, mRNA was isolated (Rneasa Mini Kit), after reverse transcription the expression was measured by quantitative RT-PCR. By almost every genes there was increase in expression after LPS stimulation in groups P+ and R+. between P- and R- the expression was same or a little bit higher in R-. R+ was allways bigger than R-. The hypothesis were that EPA and DHA should reduce expression in inflammation. This hypothesis was not proofed. I recomend further studies with the same model organism to refill the results.
|
guest ::
