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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Molecular analysis of the mitochondrial genom of \kur{Diuraphis noxia} (Aphididae)
 CHUNDELOVÁ, Daniela
The complete sequence of mitochondrial DNA from Diuraphis noxia was obtained and characterized. The mitogenome contains a standard set of 13 protein-coding genes, 19 tRNA genes, 2 ribosomal RNA genes. A+T-rich and ?repets? regions in the same order as those of the other analyzed aphids. Comparison to mtDNAs from other Sternorrhyncha species obtained from GenBank revealed possible markers for studies on population differentiation. Phylogenetic analysis using parsimony and maximum likelihood confirmed the classification of Diuraphis noxia into the Aphididae.
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Diagnostics of species complex in family Adelgidae by molecular markers.
 CHUNDELOVÁ, Daniela
Five molecular markers (mitochondrial AT-control region and COI, nuclear ITS and markers from primary enosymbiont Buchnera sp. argS and rrs) were tested for use in species identification of 2 selected genuses of the Adelgidae family. Markers COI, argS and rrs were succesfuly amplified and sequenced. Aligned sequences of those three markers were used for reconstruction of dendrogram by Neighbour-Joining metod with Kimura 2-Parameter model. These markers are suitable for resolution of taxonomic relationship of genuses within family Adelgidae, although further testing of these markers in conjunction with other markers (like ITS or AT-control region) will be required to delineate sister species with higher precision.
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