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Electro-optical sensor for the detection of extracellular ions
Bellan, Boris ; Salyk, Ota (referee) ; Vala, Martin (advisor)
The aim of this work was to prepare a platform based on electrochemical transistor that would allow both electrical and optical detection of ion exchange between the electrolyte and the layer of organic semiconductor. Semiconductor that have been used in this work was poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate). The main goal was to verify whether the proposed platform meets the requirements of both characterization methods, and whether the sensitivity in the given arrangement is sufficient for the required range of analyte concentrations. The work also dealt with the study of the stability of the prepared platform in selected solvents and culture media, at the same time the method of optimizing the preparation of the photoresist foil and the completion of the OECT measuring cell was studied. A suitable solvent for polymethyl methacrylate was sought so that it did not affect the surrounding parts of the platform. Based on the obtained results, it was found that the selected photoresist for the preparation of the spacer frame is not suitable. However, it was found that the proposed platform satisfies both characterization methods. Therefore, it will be necessary to replace the current photoresist with a new, more chemically stable one.
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Study of surface properties of PEDOT:DBSA thin film
Bellan, Boris ; Salyk, Ota (referee) ; Tumová, Šárka (advisor)
This master's thesis deals with the analysis of surface properties, with an emphasis on surface roughness and hydrophilicity of thin films prepared from pure and modified organic conjugated polymers based on poly(3,4-ethylenedioxythiophene) (PEDOT) for their potential use in bioelectronic applications. The main subject of the work was the surface analysis of the material poly(3,4-ethylenedioxythiophene):poly(dodecylbenzenesulfonate) (PEDOT:DBSA), and the influence of secondary dopants such as (3-glycidyloxypropyl)trimethoxysilane (GOPS), ethylene glycol (EG), and dodecylbenzenesulfonic acid (DBSA) on the change of surface properties of the PEDOT:DBSA polymer was also observed, with the aim of preparing a material with optimized surface properties for bioelectronic applications. In the first part of the experimental work, the surface energy of thin films was investigated by determining contact angles. In the second part of the work, the morphological properties of the surfaces of the prepared thin films were observed and compared using atomic force microscopy (AFM), scanning electron microscopy (SEM), and profilometry.
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Electro-optical sensor for the detection of extracellular ions
Bellan, Boris ; Salyk, Ota (referee) ; Vala, Martin (advisor)
The aim of this work was to prepare a platform based on electrochemical transistor that would allow both electrical and optical detection of ion exchange between the electrolyte and the layer of organic semiconductor. Semiconductor that have been used in this work was poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate). The main goal was to verify whether the proposed platform meets the requirements of both characterization methods, and whether the sensitivity in the given arrangement is sufficient for the required range of analyte concentrations. The work also dealt with the study of the stability of the prepared platform in selected solvents and culture media, at the same time the method of optimizing the preparation of the photoresist foil and the completion of the OECT measuring cell was studied. A suitable solvent for polymethyl methacrylate was sought so that it did not affect the surrounding parts of the platform. Based on the obtained results, it was found that the selected photoresist for the preparation of the spacer frame is not suitable. However, it was found that the proposed platform satisfies both characterization methods. Therefore, it will be necessary to replace the current photoresist with a new, more chemically stable one.
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