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Circadian clock genes in insects
BAZALOVÁ, Olga
This thesis focuses on molecular characterization of circadian clock genes in insects. It explores genetic diversity of circadian clock genes by molecular characterization of several insect species including two dipteran flies (Musca domestica and Drosophila melanogaster), two cockroach species representing ancestral insects, and the linden bug, Pyrrhocoris apterus. Furthermore it considers various roles of circadian clock genes in insect physiology. Application of molecular-biology methods in Pyrrhocoris apterus, non-model insect species, enable us to investigate involvement of circadian clock genes in photoperiod induced physiological responses. Application of molecular-biology methods in Periplaneta americana and Blattella germanica was used to explore involvement of circadian clock genes in magnetoreception.
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Circadian rhytm in the house fly (\kur{Musca domestica})
BAZALOVÁ, Olga
In this thesis the expression pattern of five circadian clock genes (cwo, pdp 1{$\varepsilon$}, ck 1{$\varepsilon$}, ck 2{$\beta$} and pdh) was studied in the housefly (Musca domestica). The influence of temperature on the expression pattern of these five genes and of two others genes, per and tim, was examined. The locomotor activity of flies exposed to three different temperature conditions was studied.
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Automated blood cell counting, errors and their prevention
BAZALOVÁ, Olga
One of the most commonly ordered clinical laboratory tests, a blood count, also called a complete blood count, is a basic evaluation of the cells suspended in the liquid part of the blood (plasma). Modern hematology depends on rapid and accurate determination of quantitative and qualitative blood counts. Fully automated analyzers have replaced the traditional blood smear in the routine laboratory, but the automated counting method is subject to a certain level of error. An appropriate procedure was defined, how to establish blood cell counting.
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