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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Biology and diversity of Cryptosporidium infecting squirrels (Sciuridae)
 BARVÍŘ, Pavel
In total 399 samples from three species of squirrels (Sciurus vulgaris, Sciurus carolinensis and Callosciurus finlaysonii) were collected from 2010 to 2013. All samples were examined for the presence of Cryptosporidium using by molecular methods, which demonstrated by presence of Cryptosporidium specific DNA in 18 samples (4.5%). Out of them 12 samples were positive onCryptosporidium ferret genotype, 3 on Cryptosporidium skunk genotype, 1 on Cryptosporidium chipmunk genotype I and 2 samples onCryptosporidium ubiquitum. Statistical analyses did not reveal any influence of gender on the occurrence of Cryptosporidium. Juvenile individuals of squirrels from the family Sciuridae are more often infected by Cryptosporidium than the adult animals.
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Endoparasitosis of feathered game
 BARVÍŘ, Pavel
A total of 180 samples, 119 from pheasant (Phasianus colchicus) and 61mallard (Anas platyrhynchos) were collected during two consecutive years (from 2011 to 2012). All samples were examined for the presence endoparasite using the flotation method according Sheather. In samples from pheasants were detected these types of parasites: Capillaria spp., Eimeria spp., Heterakis spp., and Trichostrongylus spp. In addition, Syngamus spp. and Cryptosporidium spp. were detected in the duck samples. Microscopical examination of aniline-carbol-methyl violet stained fecal smears revealed to Cryptosporidium spp. 12 positive samples originating from Anas platyrhynchos. DNA was extracted from Cryptosporidium positive samples and all microscopically negative samples. Nested PCR was performed to amplify the partial SSU rRNA gene of Cryptosporidium. The sequence analyses of PCR-positive specimens identified 10 samples as Cryptosporidium avian genotype III and 4 samples as C. muris. No clinical signs were detected in any Cryptosporidium positive animals. This is the first report of Cryptosporidium avian genotype III in the Czech Republic and also in Anas platyrhynchos.
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