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Evaluation of activity of potentional antibacterial substances through the use of microdilution broth method
 Švirlochová, Veronika ; Vejsová, Marcela (advisor) ; Janďourek, Ondřej (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Bc. Veronika Švirlochová Supervisor: Mgr. Marcela Vejsová, Ph.D. Diploma thesis title: Evaluation of activity of potentional antibacterial substances through the use of microdilution broth method Background The aim of this diploma thesis was to evaluate antibacterial activity of fifty-two newly synthetized substances. Methods Substances were tested through the use of microdilution broth method with eleven different concentrations. There were used eight bacterial strains and there were also used agents of nosocomial infections. Results Tested substancies were divided into seven groups by their chemical structure. The most effective group were sulphonamides. There were two groups with mininal or zero antibacterial activity. Conclusion The results showed that the most sensitive strain was Staphylococcus epidermidis and the least sensitive strain was Pseudomonas aeruginosa. There was also consideration about the presence of different substituents in different groups and their influence of antibacterial activity.
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