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HPLC method development for determination of active compounds in Arpalit preparation.
Švestková, Petra ; Šatínský, Dalibor (advisor) ; Pospíšilová, Marie (referee)
A HPLC method was developed for the separation and determination of the substances fenoxycarb and permethrin. The method is based on using HS F5 column (10 x 4 mm, 3 μm particle) and UV detection at 225 nm. The compounds were separated using isocratic elution of the mobile phase acetonitril - water (65:35) at a flow-rate of 1,0 ml/min. There was temperature 70 řC during the measurement. The system enabled successful separation of both compounds in time less than 5 min. The retention time of fenoxycarb was 1,53 min and the retention time of permethrin was 3,68 min. The chromatographic resolution between both compounds was 11,012. The method was applied to analysis of the active substances fenoxycarb and permethrin in veterinary preparations Arpalit® Neo mechanical spray, Arpalit® Neo spray a Arpalit® Neo foam. Developed method was compared with the method available on Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University in Prague (HPLC, Chromolith Performance RP-18, 100 x 4,6 mm, gradient elution of mobil phase acetonitril + water/acetonitril (60:40), flow rate 1,0 ml/min, temperature 30 řC, analysis time 11 min). Keywords: fenoxycarb, permethrin, HPLC
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HPLC Method Development for Determination of Vitamin E in Geladrink Preparation
Švestková, Petra ; Šatínský, Dalibor (advisor) ; Chocholouš, Petr (referee)
A HPLC method was developed for the determination of content of vitamine E acetate in preparation Geladrink Forte pulverized drink - pineapple. The method is based on using Chromolith Performance RP-18e column (100 x 4.6 mm) and UV detection at 290 nm. The vitamine E acetate was separated in the presence of internal standard tocol from ballast matrix. It was used isocratic elution of the mobile phase methanol:water (98:2) at a flow-rate of 1.5 ml/min. There was temperature 30 řC during the measurement. The system enabled successful separation in time less than 5 min. The retention time of tocol was 2.337 min and the retention time of vitamine E acetate was 4.271 min. The chromatographic resolution between tocol and vitamine E acetate was 7.515. The method was applied to analysis of vitamine E acetate in preparation Chondrotin - MSM 2600. Developed concentrations of vitamine E acetate were re-count on content of vitamine E that the producer declared. Keywords: vitamine E acetate, HPLC, Geladrink, Chondrotin - MSM 2600
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HPLC Method Development for Determination of Vitamin E in Geladrink Preparation
Švestková, Petra ; Chocholouš, Petr (referee) ; Šatínský, Dalibor (advisor)
A HPLC method was developed for the determination of content of vitamine E acetate in preparation Geladrink Forte pulverized drink - pineapple. The method is based on using Chromolith Performance RP-18e column (100 x 4.6 mm) and UV detection at 290 nm. The vitamine E acetate was separated in the presence of internal standard tocol from ballast matrix. It was used isocratic elution of the mobile phase methanol:water (98:2) at a flow-rate of 1.5 ml/min. There was temperature 30 řC during the measurement. The system enabled successful separation in time less than 5 min. The retention time of tocol was 2.337 min and the retention time of vitamine E acetate was 4.271 min. The chromatographic resolution between tocol and vitamine E acetate was 7.515. The method was applied to analysis of vitamine E acetate in preparation Chondrotin - MSM 2600. Developed concentrations of vitamine E acetate were re-count on content of vitamine E that the producer declared. Keywords: vitamine E acetate, HPLC, Geladrink, Chondrotin - MSM 2600
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HPLC method development for determination of active compounds in Arpalit preparation.
Švestková, Petra ; Šatínský, Dalibor (advisor) ; Pospíšilová, Marie (referee)
A HPLC method was developed for the separation and determination of the substances fenoxycarb and permethrin. The method is based on using HS F5 column (10 x 4 mm, 3 μm particle) and UV detection at 225 nm. The compounds were separated using isocratic elution of the mobile phase acetonitril - water (65:35) at a flow-rate of 1,0 ml/min. There was temperature 70 řC during the measurement. The system enabled successful separation of both compounds in time less than 5 min. The retention time of fenoxycarb was 1,53 min and the retention time of permethrin was 3,68 min. The chromatographic resolution between both compounds was 11,012. The method was applied to analysis of the active substances fenoxycarb and permethrin in veterinary preparations Arpalit® Neo mechanical spray, Arpalit® Neo spray a Arpalit® Neo foam. Developed method was compared with the method available on Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University in Prague (HPLC, Chromolith Performance RP-18, 100 x 4,6 mm, gradient elution of mobil phase acetonitril + water/acetonitril (60:40), flow rate 1,0 ml/min, temperature 30 řC, analysis time 11 min). Keywords: fenoxycarb, permethrin, HPLC
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