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Vliv vybraných faktorů na rychlost kažení plodové zeleniny
ŠUTKOVÁ, Jana
Fruiting vegetables are among the most consumed vegetables worldwide, so it is important to constantly optimise storage conditions. The aim of the thesis was to assess the effect of tomato treatments (tap water, Cibulex) and storage temperatures (6.5 °C, 15 °C, 21 °C) on the rate of spoilage, to evaluate the preferences of respondents (n=347) regarding the consumption and purchase of tomatoes and to assess the supply of tomatoes in the market network. Tomato samples treated with Cibulex solution showed a lower increase of selected groups of microorganisms compared to control samples. In the questionnaire survey, it was found that 97% of the respondents consumed tomatoes and preferred domestic cherry tomatoes, with a sweet taste and juicy consistency. When purchasing tomatoes, respondents are mainly influenced by the price, quality and appearance of tomatoes. A relatively large proportion of respondents (63 %) store tomatoes at refrigerator temperature, which is not suitable because of the loss of flavour and taste of tomatoes. A survey of the tomato offer in nine supermarket chains (Tesco, Albert, Penny, Kaufland, Globus, Flop, Coop, Norma and Lidl) revealed a wide range of tomatoes during the summer and winter periods. The widest range (n=12) was found in the Globus and Lidl chains during the winter period.
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Analysis of gonosomal mosaic using FISH method
ŠUTKOVÁ, Jana
In my bachelor thesis I dealt with the analysis of gonosomal mosaics. Mosaicism can be described as a condition in which two or more distinct cell lines originating from a single zygote are found in one organism. In women, the most common mosaic forms are Turner syndrome and triple X syndrome or superfemale. In the male population, Klinefelter syndrome and XYY syndrome (supermale) are most commonly detected. Mosaicism can be investigated by fluorescence in situ hybridization. In the theoretical part of my bachelor's thesis I focused on congenital chromosomal mosaicism, especially on its variants such as Klinefelter syndrome, Turner syndrome, triple X and XYY syndrome. Furthermore, in this part I dealt with fluorescence in situ hybridization. I described fluorescence per se and then its application in clinical practice, where several types of fluorescence in situ hybridization are used. In the practical part I examined the number of gonosomal chromosomes using fluorescence in situ hybridization. I prepared this part in the laboratory of the Faculty of Health and Social Sciences of the University of South Bohemia in České Budějovice. I examined 40 anonymized samples from 20 probands. Capillary blood samples and buccal swabs were taken from each of them. After hybridization of samples with centromeric probe for X and Y chromosomes microscopy was performed. In the last part of this bachelor thesis I dealt with the processing of the results obtained by fluorescence microscopy. Thus, a total of 6 cases of mosaicism were detected in women (the percentage of mosaic cells was above the admissible limit). In males there were a total of 2 cases of increased percentage of mosaic cells.
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