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AUXIN BINDING PROTEIN 1 (ABP1) and its role in the auxin management in plant cells
Čovanová, Milada ; Zažímalová, Eva (advisor) ; Lüthen, Hartwig (referee) ; Reinöhl, Vilém (referee)
Conclusions The role of AUXIN BINDING PROTEIN 1 (ABP1) in the auxin management in plant cells was followed using simplified model material of suspension-cultured cells of tobacco BY-2 line. ABP1 is a putative auxin receptor considered to mediate fast non-genomic responses to auxin and it can be involved in every aspect of the regulation of auxin responses, metabolism and transport. There are four major conclusions that could be made based on the results presented in this thesis: 1) Auxin binding protein 1 mediates both cell division and expansion in tobacco BY-2 cells. In standard cultivation conditions or at lower concentrations of 2,4-D in culture medium, ABP1 overexpression had no detectable impact on cell division, cell elongation or cell growth.. 5- times increased 2,4-D concentration stimulated weakly cell elongation. . Antisense suppression of ABP1 expression resulted in disturbance in both cell expansion and cell division intensity, suggesting that ABP1 is essential for the control of balance between cell division and cell elongation during the growth cycle. ABP1 is localized in endoplasmic reticulum of cells cultivated in standard medium supplemented with 1 μM 2,4-D and it appeared also at the plasma membrane following the IAA application. 2) ABP1 mediates intercellular auxin transport. Cells...
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AUXIN BINDING PROTEIN 1 (ABP1) and its role in the auxin management in plant cells
Čovanová, Milada ; Zažímalová, Eva (advisor) ; Lüthen, Hartwig (referee) ; Reinöhl, Vilém (referee)
Conclusions The role of AUXIN BINDING PROTEIN 1 (ABP1) in the auxin management in plant cells was followed using simplified model material of suspension-cultured cells of tobacco BY-2 line. ABP1 is a putative auxin receptor considered to mediate fast non-genomic responses to auxin and it can be involved in every aspect of the regulation of auxin responses, metabolism and transport. There are four major conclusions that could be made based on the results presented in this thesis: 1) Auxin binding protein 1 mediates both cell division and expansion in tobacco BY-2 cells. In standard cultivation conditions or at lower concentrations of 2,4-D in culture medium, ABP1 overexpression had no detectable impact on cell division, cell elongation or cell growth.. 5- times increased 2,4-D concentration stimulated weakly cell elongation. . Antisense suppression of ABP1 expression resulted in disturbance in both cell expansion and cell division intensity, suggesting that ABP1 is essential for the control of balance between cell division and cell elongation during the growth cycle. ABP1 is localized in endoplasmic reticulum of cells cultivated in standard medium supplemented with 1 μM 2,4-D and it appeared also at the plasma membrane following the IAA application. 2) ABP1 mediates intercellular auxin transport. Cells...
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