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Electrophoretic mobility measurements of a multivalent red dye SPADNS
Pantůčková, Pavla ; Kubáň, Pavel ; Boček, Petr
Measurements of the electrophoretic mobility of an anionic dye 4,5-dihydroxy-3-(p-sulfophenylazo)-2,7- naphthalene disulfonic acid trisodium salt (SPADNS), which is a commonly used anionic dye, are described in this contribution. Electrophoretic mobilities of SPADNS were measured in various buffer solutions covering the pH range of 2.4–9.1. Effective electrophoretic mobilities of SPADNS measured in these buffers varied between 50.5 x 10–9 m2/Vs and 60.5 x 10–9 m2/Vs with an average effective mobility of 55.0 x 10–9 m2/Vs.
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Preparation of specific probes detecting prophages of serogroups A,B and F in lysogenic strains of Staphylococcus Aureus
Doškař, J. ; Pallová, P. ; Pantůček, R. ; Rosypal, S. ; Růžičková, V. ; Pantůčková, Pavla ; Kailerová, J. ; Klepárník, Karel ; Malá, Zdeňka ; Boček, Petr
On the basis of HindIII-restriction digest analysis of genomic DNAs, the S. aureus bacteriophages of the International Typing Set were divided into five clusters (A, F, Ba, Bb, and Bc). For each of the phage serogoups A, B, and F, common HindIII-restriction fragments of 3A, 53 and 77 phages were used for preparation of probes specific to the phages of all serogroups. These probes enable to detect up to three different prophages in a given lysogenic strain at the same time. Restriction enzyme maps of phages 3A, 53, and 77, each representing a different serogroup, were constructed.
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