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Immunologic Characteristics of Cord Blood in Children with Increased Risk of Allergy Development Preventive Use of Probiotics
Hrdý, Jiří ; Prokešová, Ludmila (advisor) ; Tlaskalová - Hogenová, Helena (referee) ; Ulčová-Gallová, Zdeňka (referee)
Allergy is one of the most common diseases. Identification of early prognostic markers pointing to an increased risk of allergy development is therefore of increasing importance. Cord blood represents an easily attainable clinical material for searching for prognostic markers signalizing future allergy development. Proportions of Th1 cytokines, Th2 cytokines and regulatory cytokines were tested in cord blood of children of allergic mothers (children in relatively high risk of allergy development) in comparison with cord blood of children of healthy mothers (low risk children). Also the activities of lymphocytes, dendritic cells (DC) and regulatory cells (Tregs) were compared in children of healthy and allergic mothers. The generally increased activity of both in vitro stimulated and non-stimulated mononuclear cord blood leukocytes was proved in children of allergic mothers in comparison with low risk children. The increased activity of DC of high risk children was detectable only after polyclonal stimulation. Significantly less pronounced functional properties of cord blood Tregs were found in children of allergic mothers when compared with children of healthy mothers. The increased reactivity of lymphocytes and DC together with the decreased activity of Tregs can support an easier...
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Proteomics of biological fluids
Jarkovská, Karla ; Kovářová, Hana (advisor) ; Petrák, Jiří (referee) ; Ulčová-Gallová, Zdeňka (referee)
Reproductive diseases, mainly those resulting in the infertility affect the chances of human being to reproduce. On the contrary, the heart disease, cancer and degenerative diseases currently account for majority of deaths in the world. Usually, these lifestyle diseases need longer lifespan to become the cause of death. The proteins secreted by cells carry important information about the cell's well-being, as well as about the condition of the tissues formed by these cells. Once secreted, these proteins may also be transferred throughout the body by means of body fluids, many of which are easily accessible for further 'in-depth' studies. Cellular and secreted proteins are often a focus of studies using proteomic means and the revelation of protein alterations can lead us to new ideas about the molecular mechanisms of diseases as well as possible identification of proteins that may be used as new targets for pharmaceutical intervention or molecules that could be used for diagnostic or prognostic purposes. Taking into consideration the above aspects, this research was undertaken to find proteins that could: (a) characterise the human follicular fluid as microenvironment of the maturing oocyte, to increase understanding of reproductive processes to improve the techniques of assisted repro- duction;...
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Preeclamsia and selected immunogenetic factors
Hradecký, Libor ; Ulčová-Gallová, Zdeňka (advisor) ; Madar, Jindřich (referee) ; Oborná, Ivana (referee)
Preeclampsia and selected immunogenetic factors Our objective was to evaluate plasma levels of the eight most common antiphospholipid antibodies (antiphosphatidylserine, antiphosphatidylethanolamine, antiphosphatidylinositol, antiphosphatidylglycerol, antiphosphatidic acid, antiannexin V, anticardiolipin and anti 2-glycoprotein I antibodies) by ELISA method and selected inherited thrombophilia (F V- Leiden mutation, FII mutation G20210A, C677T and A1298C variants of the gene for methylene tetrahydrofolate reductase-MTHFR) by DNA analysis of peripheral blood lymphocytes using the real-time PCR in fifty-five women with preeclampsia in the period immediately before urgent termination of pregnancy. Fifty-five healthy women without preeclampsia was considered as a controll group. Entered data were examined using a non-parametric Wilcoxon's test, univariate analysis were perfomed using the Fisher's exact test and statistical dependence between variables was assessed using Spearman's rank correlation coefficient. We demonstrated that women with preeclampsia had significantly higher levels of anticardiolipin antibodies in the isotope IgG (p <0.01) and IgM (p <0.01), elevated levels of antiphosphatidylserine antibodies in the isotope IgG(p <0.01) and antiethanolamine antibodies in the isotope IgM (p <0.01) when...
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Autoimmune ovarian damage in adolescent girls, the impact antiovarian antibodies to irregular menstrual cycles and fertility
Kosová, Hana ; Hořejší, Jan (advisor) ; Grim, Miloš (referee) ; Fučíková, Terezie (referee) ; Ulčová-Gallová, Zdeňka (referee)
pou~i II "Iaylnr madt: Ihcra ", ledy pouá\ <iní malých, kontrolm':lné individuálních djvek lmg"nú. mbJcuovaných po I. ji ~ li:' lIf pOLdní pl'<Jlilerac \ honnonálni fun kč n í cytol(lgii rroge~lt:rnnCIl1 O ú...pé hu na~ í terapie jsme se pfcw~ dč iJi pn detekl: hladin) antio\'ariálních protilmek. kdy Isme v 40. 16 r l<unamell"li ...níLení a u J ().9~'k pac'ientck ymiLenl protiljtek. lento rakt je Jol ,zen i tim. ze u , ě l šiny paci nt k pi'i této terapii se objekt i vně I.lcpšil ,tav vnitr'ních rodidel, seKundární h p0hlavn ích znakú . Za zcela zjsadoí PO\ 37Ukjc "Ol/čas né Lkpšení koslní d' n, i t~" 8, SHRNUTÍ );,Iedky naší studie a mapování výskytu sérových anlio\'ari:ílních prulil51c:k mis vcde k přesvědče n í. ze výskyt autoprotilátek proli ovariu kore<;pondujc , dříve č i pOldiíji ,e prclcntu jícími klinickými přízn ak y . On ' mocnění múže propuknou v obdohí od puherty po celé reprod u kč ní období. Zavedení adekvátní léč by (v na' i studi i s ' osvědčilo použití HRT) podle našich poznatku vedlo ke snížení autoimunního ro ~ kole ní ovaria na minimum, k L:lchování jeho hormonáln ích funkcí a pIedevším k zachování zdl'avé ovariální tkáně, n.:Lb)lné pro budoucí rertilitu pacientky. Naše tudie tedy potvrdila nepochyhně obrovsky význam vča s n é diagnostiky a léčb y autoimunitního one muc ně ní uvaria, Z...
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