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In vitro models of skin barrier
Šimek, Matěj ; Zbytovská, Jarmila (advisor) ; Pullmannová, Petra (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Candidate: Matěj Šimek Consultant: doc. Dr. rer. nat. Mgr. Jarmila Zbytovská Title of thesis: In vitro models of skin barrier The aim of this work is to summarize information about various types of skin models which are used for testing of permeability, toxicity, irritability and other aspects of drugs, through professional, verified and reviewed literature. These characteristics are necessary to know in order to grant optimal safety, effectiveness nad quality of transdermally administered drugs. Transdermal administration of drugs has got lots of benefits in contrast with classic peroral administration. An administration of drugs through this way is quite simple and it can be interrupted quite easily. We can also easily change a place of administration in which a drug is released and the risk of overdosing is very low. Furthermore, transdermal administration makes possible to maintain constant plasmatic concentration of drug in a blood stream and also to prolong the duration of effect of drugs with small halftime thanks to constant releasing of drug. And primarily, transdermally administered drug normally avoids the "first-pass" effect of liver, so the dose of drug can be lowered. The risk of drug...
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The effect of cosmetic products with different pH on the skin properties of men and women
Drabová, Veronika ; Kuchařová, Monika (advisor) ; Pullmannová, Petra (referee)
The aim of this diploma thesis was to summarize the basic knowledge of human skin and it's biophysical parameters. Attention was focused on influence of cosmetic products with different pH on the change of skin parameters especially. The first part describes the anatomical and histological structure of the skin, it's basic functions and development. In the following chapters of this work is mentioned physiological representation of microorganisms found on the skin surface and general characteristics of cosmetic products. There are used in everyday skin care that affect the natural skin surface pH value. Optimum surface pH is important for the proper functioning of human skin. The final chapters of the described the characteristics of the basic biophysical parameters of the skin (hydration, TEWL, pH, sebum) and methods for their measurement. Key words: skin, transepidermal water loss, pH, hydration, sebum, microbiome
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Synthesis of phytoceramide analogues and evaluation of their effects on the skin barrier properties
Tesař, Ondřej ; Vávrová, Kateřina (advisor) ; Pullmannová, Petra (referee)
Charles University in Prague Faculty of Pharmacy in Hradci Králové Deparment of Inorganic and Organic Chemistry Candidate: Ondřej Tesař Supervisor: doc. PharmDr. Kateřina Vávrová, Ph.D. Title of diploma thesis: Synthesis of phytoceramide analogues and evaluation of their effect on the skin barrier properties. Phytoceramides (FCER) belong to the class of sphingolipids. They are composed of a basic alcohol phytosphingosine (P) and the acyl chain. Together with the other lipids they create the extracellular membrane in human stratum corneum (SC), the uppermost layer of the skin, which protects us against the loss of water and penetration of foreign compounds into our body. The influence of FCER structure on the function of this membrane is unknown yet. Aim of the work: The aim of this work was to examine what role the acyl chain length of FCER plays in this membrane impermeability. Methods: My task was to synthetize FCER with acyl chain length of 2, 4, 6 and 24 carbons and preparation of SC model membranes containing FCER with acyl chain length of 2, 4, 6, 8 or 24 carbons, cholesterol, lignoceric acid and cholesterol sulfate. The permeabilities of the model membranes were evaluated in Franz diffusion cells. Electric impedance and permeability for two model drugs: theophylline (TH) and indomethacin...
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