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National Repository of Grey Literature 12 records found  previous11 - 12  jump to record: Search took 0.00 seconds. 
Structure and evolution of efflux pump in gramnegative bacteria.
 Gálová, Diana ; Lichá, Irena (advisor) ; Matyska Lišková, Petra (referee)
Drug resistence in microorganisms is of major concern and there is an increasing number of pathogenic bacteria resistent to clinically used antibiotics. The major mechanism of resistence in Gram-negative is active efflux that prevent the intracellular accumulation of antibiotics to toxic levels. We recognise five families of efflux pumps, mainly using proton motive force to translocate the substrate, less common driven by ATP hydrolysis. The multidrug pumps with broad substrate specifity are of a key role in drug resistance of pathogens, typically represented by RND superfamily. The source of efflux pumps are probably the producers of antibiotics and organisms exposed to toxic compounds in their natural habitat. The genes for resistance can then spread out via horizontal gene transfer to other bacteria. It is presumed that eflux pumps have developed from transporters serving physiological functions like transport of endogenous substrates.
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Microbial communities of soils affected by long-term heavy metal contamination
 Průchová, Pavla ; Kopecký, Jan (advisor) ; Matyska Lišková, Petra (referee)
This work is focused on microbial communities living in the soil affected by long-term exposure to heavy metals. The soil was sampled at two sites with different levels of contamination near Příbram. In the samples, respiration rate was measured in vitro after addition of carbon sources and at different levels of cadmium, one of the contaminating metals found in the soil. After the incubation with carbon sources, soil samples were collected for environmental DNA isolation. Gene coding for 16S rRNA in Actinobacteria was amplified from the environmental DNA samples and the amplicon composition was assessed by terminal restriction fragment length polymorphism analysis. The resulting profiles were used to compare actinobacterial communities in both groups of soil samples and in individual treatments. The analysis showed a clear distinction between the two sites differing in the contamination level and shifts in the community composition of various intesity depending on the added substrate.
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National Repository of Grey Literature : 12 records found   previous11 - 12  jump to record:
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