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Preparation of cochenial carmine
Helceletová, Zuzana ; Hrdličková Kučková, Štěpánka (advisor) ; Čapek Adamec, Martin (referee)
The aim of this study was to prepare a red organic pigment named cochineal carmine. The pigment was made out of dried insect called Coccus cacti using historical recipe. Next goal was to detect carminic acid by infrared spectroscopy with Fourier transform (FTIR) in dried bodies of the mentioned insect, then in the pigment prepared from it and in the pigment that was prepared using extract called carminum rubrum. The other results of this work were the comprehension of the origin and preparation of carmine pigment and the identification of carminic acid in both prepared pigments.
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Iris green
Menclová, Kateřina ; Hrdličková Kučková, Štěpánka (advisor) ; Crhová, Michaela (referee)
The goal of this thesis was to summarize available information about natural green organic dyes. This specific group of dyes is not too deep by itself; therefore other groups of natural dyes with a description of their chemical structures, natural resources and historical utilization, were also added. The work is aimed for preparation of organic dye that is called iridaceous green according to medieval recipes. This green dye was prepared from blue iris blows and than white linen was dyed by the plant extract. The dye together with the linen serve as a reference samples for future identification of natural organic dyes.
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Identification of milk and collagenous binders used in colour layers in art works
Kofroňová, Pavla ; Hrdličková Kučková, Štěpánka (advisor) ; Čapek Adamec, Martin (referee)
The task of this diploma thesis was to investigate the influence of inorganic pigments on the identification of casein oil and animal glue tempera by mass spectrometry MALDI- -TOF MS (Matrix-Assisted Laser Desorption/Ionisation - Time of Flight Mass Spectrometry). First, it was necessary to prepare a set of model colour layers containing casein and animal glue tempera together with ten inorganic pigments. Consequently, the method of peptide mass fingerprinting was used to obtain mass spectra of the samples. Finally, it was possible to compare and determine the characteristic peptide fragments for both proteinaceous binders and find pigments that most affected the identification of casein and rabbit glue temperas. The obtained values of m/z casein oil tempera and animal glue tempera were used to improve and complete reference database of protein binders that is used to identify protein binders in works of art at the Institute of Chemical Technology in Prague.
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Differentiation of different types of eggs by mass spectrometry
Švárová, Markéta ; Kučková, Štěpánka (advisor) ; Rusek, Martin (referee)
The aim of the diploma thesis was to distinguish bird species based on the expected different protein composition of eggs and their individual components using mass spectrometry. The obtained results could be possibly used to identify the animal origin of egg materials used in works of art. For the research, components of the eggs (yolk, egg white and their mixture) of nine available bird species were collected and dried - pheasant (Phasianus colchicus), domestic goose (Anser anser domesticus), domestic duck (Anas platyrhynchos domesticus), muscovy duck (Cairina moschata), chicken (Gallus gallus f. domestica) - four different specimens, Japanese quail (Coturnix japonica), pampas rhea (Rhea americana), red ore (Alectoris rufa), speckled guinea fowl (Numida meleagris). The samples were digested by the enzyme trypsin and prepared using ZIP-TIP for the next measurements by MALDI-TOF (Matrix-Assisted Laser Desorption / Ionization - Time of Flight) mass spectrometry. The obtained data were evaluated by Principal Component Analysis (PCA). The used method showed that the most bird species can be distinguished by yolks (with an approximately 95% success rate) and more than half of the species by egg white proteins (success rate 83%) and by the egg white and yolk mixtures (success rate 80%). Two samples of...
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Proteomic differentiation of hairs of different dog breeds
Humpoláková, Karin ; Kučková, Štěpánka (advisor) ; Kolář, Karel (referee)
The goal of my thesis was to find out whether it is possible to distinguish among individual dog breeds from the protein analysis of their fur. This knowledge could be used, for example, in forensic science. In this work, there was also a comparison of the similarity of dog breeds with a wolf, which was domesticated and is considered as an ancestor of the dog. For this study, the hair of three representatives of sixteen dog breeds was collected. To analyse these samples enzyme cleavage was used a trypsin, and mass spectra were obtained by MALDI- TOF MS (Matrix-Assisted Laser Desorption/Ionization - Time of Flight Mass Spectrometry) and LC/MS-MS (Liquid Chromatography with Tandem Mass Spectrometry) methods. The obtained data were evaluated by the PCA (Principal Component Analysis) and LDA (Linear Discriminant Analysis) method. It was found, that individual dog breeds cannot be distinguished using both methods. KEYWORDS proteomics, coat, dog, breeds, mass spectrometry
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